高效液相色谱-串联质谱法鉴定伊维菌素的成分  

Identification of Ivermectins by High Performance Liquid Chromatography-Electrospray-Mass Spectrometry-Mass Spectrometry

作　　者：雷荣[1] 马丽娟 怀其勇[1] 左育民[1] 

机构地区：[1]天津南开大学化学系,天津300071 [2]石家庄华晟生物化工厂,石家庄050000

出　　处：《分析化学》2002年第1期26-30,共5页Chinese Journal of Analytical Chemistry

摘　　要：利用高效液相色谱从商品化伊维菌素中分离出８种组分，再与ＥＳＩ－ＭＳ－ＭＳ偶合，得到其加合离子和特征子离子，从而对各组分的化学成分进行了鉴定。报道了伊维菌素Ｂｌａ的１３Ｃ ＮＭＲ谱图，并对碳信号作了归属。8 fractions of commercial ivermectin were isolated and identified by high performance liquid chromatography-electrospray-mass spectrometry-mass spectrometry (HPLC-ESI-MS-MS). The molecular ions themselves were not seen in the ESI-MS spectra, but adduct ions such as [M + Na](+) ions were prominent. The MS-MS data of HPLC fractions were also obtained by the ESI-triple quadrapole mass spectrometer. The charaeteristic daughter ions could be used to distinguish the same molecular weight components. The C-13 nuclear magnetic resonance (NMR) spectrum of ivermectin B-1a was reported and explained with its structure.

关 键 词：伊维菌素 阿维菌素 高效液相色谱 串联质谱 ^13C核磁共振谱 鉴定 兽药 化学成分 分析 

分 类 号：O657.63[理学—分析化学]