过硫酸钾存在下人血清白蛋白的极谱平行催化氢波及其应用  

Parallel Catalytic Hydrogen Wave of Human Serum Albumin in the Presence of Potassium Persulfate

作　　者：刘利民[1] 过玮[1] 宋俊峰[1] 

机构地区：[1]西北大学化学系

出　　处：《分析化学》2002年第1期46-49,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.29875017)

摘　　要：报道 Ｋ２Ｓ２Ｏ８存在下人血清白蛋白（ＨＳＡ）的极谱平行催化氢波。在 ０．４ ｍｏｌ／Ｌ　ＮＨ３－ＮＨ４Ｃｌ（ｐＨ ８．５８）－０．０１ ｍｏｌ／Ｌ Ｋ２ｓ２Ｏ８支持电解质中，ＨＳＡ产生一个平行催化氢波，峰电位为－１．８５ Ｖ（ｖｓＡｇ／ＡｇＣｌ）。该平行催化氢波二阶导数峰电流与 ＨＳＡ浓度在 ２．６ × １０－９～９．６ × １０－９ｍｏｌ／Ｌ范围呈线性关系。用于人血清样和尿样中ＨＳＡ的测定，结果满意。A new kinetic wave, termed as parallel catalytic hydrogen wave, of human serum albumin (HSA) in the presence of potassium persulfate was observed. In 0.4 mol/L NH3-NH4Cl (pH 8.58)-0.01 mol/L K2S2O8 supporting electrolyte, HSA yielded a parallel catalytic hydrogen wave with peak potential of -1.85 V ( vs. Ag/AgCl). The second-order derivative peak current of the parallel catalytic hydrogen wave was linearly proportional to HSA concentration in the range of 3.2 x 10(-9) similar to 9.6 x 10(-9) mol/L. The parallel catalytic hydrogen wave was applied to the determination of HSA content in human serum and urine with satisfactory results.

关 键 词：人血清白蛋白 蛋白质 极谱平行催化氢波 过硫酸钾 测定 血清 尿样 

分 类 号：O657.14[理学—分析化学]