测定利福平的化学发光新体系  

A Chemiluminescence Method for the Determination of Rifampicin

作　　者：刘伟[1] 李保新[1] 章竹君[1] 

机构地区：[1]陕西师范大学化学系,西安710062

出　　处：《分析化学》2002年第1期86-88,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.39730160)

摘　　要：基于碱性介质中铜离子催化过氧化氢可氧化利福平产生化学发光这一现象，并结合流动注射分析技术建立起了一种测定利福平的新方法。该方法测定利福平的线性范围为 ４ × １０－７× １０－４ｇ／ｍｌ，方法的检出限为 ７× １０－８ｇ／ｍＬ，７次平行测定 ２ × １０－５ｇ／ｍＬ利福平的相对标准偏差为 ４．９％。成功地用于利福平眼药水中利福平含量的测定。Flow-injection chemiluminescence (CL) for the determination of rifampicin was suggested. It was based on the CL reaction of hydrogen peroxide and rifampicin in the presence of Cu2+, as catalyst, in basic medium. The CL intensity was linear with rifampicin concentration in the range of 4 x 10(-7) similar to 1 x 10(-4) g/mL. The relative standard deviations for 2 x 10(-5) g/mL rifampicin (n=7) was 4.9%. The detection limit was 7 x 10(-8) g/mL. This method was used to determine rifampicin in rifampicin ocustilla with satisfactory results.

关 键 词：化学发光 流动注射 药物分析 利福平 抗生素 眼科药物 眼药水 测定 

分 类 号：O657.3[理学—分析化学]