测定脑脊液中兴奋性氨基酸的样品前处理方法  

The Effect of Different Pretreatment on the Determination of Excitatory Amino Acids in Cerebrospinal Fluid Sample

作　　者：张虹[1] 左玫[2] 梁琪[2] 李永民[1] 陈立仁[1] 

机构地区：[1]中国科学院兰州化学物理研究所,兰州730000 [2]兰州医学院第一附属医院,兰州730000

出　　处：《分析化学》2002年第1期97-100,共4页Chinese Journal of Analytical Chemistry

摘　　要：采用高效液相色谱法，研究脑脊液样品不同的前处理方法对脑脊液中兴奋性氨基酸（谷氨酸、天门冬氨酶）测定结果的影响。结果表明：强酸性蛋白沉淀剂可导致脑脊液中的谷氨酸异常增高，脑脊液贮存于－８０℃并以甲醇作为蛋白沉淀剂时，兴奋氨基酸稳定，测定结果不变；如果脑脊液贮存在－２０℃，不能超过８周。建立了准确、灵敏的兴奋性氨基酸分析方法，为临床拮抗兴奋性毒性和进一步深入研究兴奋性氨基酸提供可靠的测定方法。The changes of excitatory amino acids (EAAs, including glutamate and aspartate), in cerebrospinal fluid (CSF) were investigated by of different pretreatment methods. High performance liquid chromatography was employed for the determination of EA-As. It was found that deproteinization by precipitation with strong acid could lead to falsely high measurements of glutamate and when CSF was stored at -80degreesC and deproteinized with methanol before assay the EAAs in cerebrospinal fluid kept constant. It was suggested that the stored time of the sample could not be longer than 8 week when the cerebrospinal fluid was stored only at -20degreesC. An accurate and sensitive reversed-phase high performance liquid chromatographic method has been developed for anti-excitotoxity therapy and thorough study of EA-As in clinical.

关 键 词：高效液相色谱法 脑脊液 谷氨酸 天门冬氨酸 氨基酸 样品前处理 兴奋性毒性 脑疾病 

分 类 号：O657.7[理学—分析化学]