反义c-myc涂层支架对兔颈动脉细胞凋亡的影响  

Effect of stent absorbed c-myc antisense oligodeoxynucleotide on smooth muscle cells apoptosis in rabbit carotid artery

作　　者：张新霞[1] 崔长琮[1] 李江[2] 孟猛[1] 徐仓宝[3] 赵一岭[2] 

机构地区：[1]西安交通大学第一医院心内科 [2]第四军医大学基础部病理学教研室 [3]西安交通大学医学校区动脉粥样硬化研究室

出　　处：《临床心血管病杂志》2001年第12期562-564,共3页Journal of Clinical Cardiology

基　　金：陕西省科技研究发展计划项目 (No .99K13 G8)

摘　　要：目的 :探讨铂 铱合金明胶蛋白涂层支架局部导入c myc反义寡核苷酸 (ASODN)对兔颈动脉细胞凋亡的影响 ,寻求防治支架内再狭窄的途径。方法 :将携带c mycASODN的国产铂 铱合金明胶蛋白涂层支架置入兔颈动脉 (给药组 ,n =16 ) ,在术后 7、14、30、90d处死动物行苏木精 伊红和Weigert染色 ,图像分析测量新生内膜厚度和面积 ,c myc蛋白免疫组化染色 ,采用末端脱氧核苷酸酶介导的dUTP缺口末端标记法检测细胞凋亡 ,并与对照组 (n =16 )进行对比分析。结果 :两组支架术后 7、14d均未观察到平滑肌细胞凋亡 ,术后 30d在新生内膜中观察到明显的细胞凋亡 ,90d时显著高于 30d ;同时给药组平滑肌细胞的凋亡显著高于对照组。结论 :c mycASODN可诱导支架置入后平滑肌细胞凋亡 。Objective:To investigate the effect of gelatin coated platinium iridium stent absorbed c myc antisense oligodeoxynucleotide (ASODN) on smooth muscle cells apoptosis in a normal rabbit carotid arteries.Method:Gelatin coated platinium iridium stent were implanted in the right carotid arteries of 32 rabbits under vision. Animals were randomized into the control group and the treated group receiving c myc ASODN (n=16 respectively).On the 7, 14, 30 and 90 days following the stenting procedure. morphometry for calculation of neointimal area and mean neointimal thickness were performed.The expression of c myc protein was detected by immunohistochemical methods.Apoptotic smooth muscle cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL) .Result:During 7 and 14 days after stenting, there were no detectable apoptotic cells in both groups. The apoptotic cells occurred in the neointima 30 and 90 days after stenting, and the percentage of apoptotic cells was less on 30 days than on 90 days. Meanwhile, c myc ASODN induced more apoptotic cells in treated group than that in control group. Conclusion:C myc ASODN can induce smooth muscle cells apoptosis after stenting in normal rabbit carotid arteries, and it can be used to prevent in stent restenosis.

关 键 词：细胞凋亡 平滑肌细胞 再狭窄 支架 C-MYC反义寡核苷酸 ASODN 颈动脉 

分 类 号：R543.4[医药卫生—心血管疾病]