不同的冻存方法对脐血CD34^+细胞的影响  

Recovery of CD34 Cells After Cryopreservation of Umbilical Cord Blood Cells in Three Different Methods

作　　者：谢燕[1] 敖述斌[1] 夏虹[1] 高清平[1] 李秀珍[1] 

机构地区：[1]武汉大学人民医院血液科,武汉430061

出　　处：《武汉大学学报（医学版）》2001年第2期153-155,共3页Medical Journal of Wuhan University

基　　金：荷兰皇家科学院和国家科委 (KNAW SSTC项目号 96CD0 46 )部分资助

摘　　要：目的 :研究不同冻存方法对脐血CD34+ 细胞的影响 ,探讨脐血CD34+ 细胞先分离后冻存的可能性 ,以寻找脐血保存的更好方法。方法 :对 31份脐血采用全脐血直接冻存 ,分离为单个核细胞冻存 ,和分离为CD34+ 细胞冻存 ,3种不同的冻存方法 ;用造血祖细胞培养技术、活细胞染色技术、流式细胞技术等方法研究冻存前后脐血造血祖细胞活性的变化。结果 :冻存后细胞活度下降 ,集落形成率下降 ,以全脐血冻存组为甚 ;CD34+ CD38- 比例冻存后增高。结论 :先分离后冻存法可行 ,且具有减小冻存体积 ,对造血祖细胞损伤较小的特点 ;Objective:To study the effect of umbilical cord blood(UCB) transplantation, and combination of UCB transplantation and gene therapy, one of major therapy for hereditary hematological disease in the future.Cryopreservation of UCB was critical for the use of UCB.This experiment was to optimize the procedure of cryopreservation.Methods:Three different cryopreservation procedures-whole UCB,mononucleate cells' suspension and CD34 + cells' suspension were used in this study.Recovery of CD34 + cells was evaluated by viability assay,clonogenic capacity assay and percentage of CD34 + cell assay.Results:The viability and clonogenic capacity were decreased after cryopreservation,especially cryopreservation of whole UCB directly.The percentage of CD34 +CD38 - cells was increased after cryopreservation.Conclusion:①Cryopreservation after separation is feasible.If CD34 + cells are going to be separated by magnetic system,cryopreservation after separation will not only reduce the storage volume but also lead to less damage of CD34 +.②The early hematopoietic progenitors might be more tolerance to the cryopreservation.

关 键 词：胎血 冷冻保存 CD34^+细胞 造血干细胞移植 

分 类 号：R457[医药卫生—治疗学] R318.52[医药卫生—临床医学]