吸毒人群中庚肝病毒与乙肝病毒基因的同步检测  

Synchronous gene detection of HGV and HBV among drug users

作　　者：伍欣星[1] 郑义[1] 赵旻[1] 谭云[1] 戴天力[1] 

机构地区：[1]武汉大学医学院病毒研究所分子生物学研究室,湖北武汉430071

出　　处：《中国实验诊断学》2001年第6期285-287,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的 　调查吸毒人群中肝炎病毒感染状况，探讨肝炎病毒之间的相互关系以及庚肝病毒感染的临床意义。方法 从血清中同步提取 ＲＮＡ和 ＤＮＡ，ＲＴ－ＰＣＲ扩增 ＨＧＶＲＡ，多重 ＰＣＲ扩增 ＨＧＶ ｃＤＮＡ和 ＨＢＶ ＤＮＡ。ｘ２检验进行统计学分析。结果 吸毒人群中 ＨＧＶ感染率为 ３９．６％、ＨＢＶ ３１．１％、ＨＧＶ与 ＨＢＶ合并感染率为 １１．３％；ＨＧＶ－ＲＮＡ阳性和ＨＧＶ－ＲＮＡ／ＨＢＶＤＮＡ阳性者，ＡＬＴ平均值分别为４６　Ｕ／Ｌ和４７　Ｕ／Ｌ；与全阴性人比较有显著性差异（Ｐ＜０．０１）。结论①建立了一种基因检测方法，可同步检测ＤＮＡ病毒和ＲＮＡ病毒。②吸毒人群ＨＧＶ感染率高，其单独感染或与ＨＢＶ合并感染对ＡＬＴ改变有一定影响。但ＨＧＶ与肝病的关系需要进一步资料积累。Objective To investigate the infection of hepatitis and relation between virus among drug users, to probe the clinic significance 0f HGV infection. Methods DNA and RNA were synchro-extract from bloxl. RT-PCR was used to amplify HGV. HGV eDNA and HBV DNA were synchro-deteted by multi-PCR. Statistical analysis (Shi-square teste) was made in clinical. Re- sults HGV-RNA and HBV-DNA were quickly synchroextracted and gene detected. The infection rate of HGV was 39.6%; HBV was 31.1%. Together infection rate of HGV and HBV was 11.3%, ALT medians were 46 U/L and 47 U/b respectively in only people of HGV pcnitive and HGV/HBV xnitive people. Compared with the negative people lxth had significant difference (P< 0. 01). Conclusion ①A method of DNA and RNA synchrony-detection was established. ②The infection rate of HGV alone and co infection were higher in drug user. ALT levels had effected a litter for HGV alone and co infection. But the relation of HGV and hepatitis needs further certificate.

关 键 词：庚肝病毒 基因 同步检测 吸毒者 HBV DNA RNA HGV 

分 类 号：R446.5[医药卫生—诊断学]