复方蛇床子洗剂中蛇床子素的RP-HPLC测定  

Determination of osthol in LOTIO FRUCTUS CNIDII COMPOSITA by RP-HPLC

作　　者：刘新宇[1] 吴芳[1] 范国荣[2] 胡晋红[2] 

机构地区：[1]深圳市福田区人民医院药剂科,广东深圳518033 [2]上海市长海医院药材科,上海200433

出　　处：《中草药》2001年第9期790-793,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的　建立复方蛇床子洗剂中蛇床子素定量分析的 RP- HPL C方法。方法　选择醋酸甲地孕酮为定量内标物 ,采用 Hypersil- ODS2 (2 0 0 mm× 4.0 m m,5μm )色谱柱 ,流动相为 0 .0 1m ol/L磷酸二氢钠 -乙腈 (5 2∶ 48) ,流速1.2 m L/min,检测波长 32 0 nm。结果　蛇床子素和内标在 12 min之内实现理想分离。在 0 .12 3 8～ 2 .970 μg/m L范围内 ,蛇床子素与内标峰面积比与其相应浓度呈良好的线性关系 (r=0 .9996 ) ,方法平均回收率为 (98.4±0 .90 ) % ,RSD为 0 .91%。结论　本法操作简便快速 ,定量准确可靠 ,可用于复方蛇床子洗剂的质量控制。Object To estabish a method for the quantitative analysis of osthol in LOTIO FRUCTUS CNIDII COMPOSITA by RP HPLC Methods Megestrol acetate was used as the internal standard Results Chromatographic separation of osthol and megestrol acetate was accomplished within 12 min on Hypersil ODS 2(200 mm× 4 0 mm, 5 μm) column and acetonitrile 0 01 mol/L sodium dihydrogen phosphate (48∶52) as mobile phase The flow rate was 1 2 mL/min and the detection wavelength was 320 nm A good linearity was obtained in the range of 0 123 8～2 970 μg/mL (r=0 999 6) for osthol and the average method recovery was (98 4±0 90) % with RSD=0 91% Conclusion The method was simple, rapid, accurate, reliable, and suitable for the quality control of LOTIO FRUCTUS CNIDII COMPOSITA

关 键 词：复方蛇床子洗剂 蛇床子 蛇床子素 RP-HPLC 中药 

分 类 号：R286.0[医药卫生—中药学]