失血性休克大鼠血浆及腹腔巨噬细胞释放细胞因子改变  

The change of cytokines in the peritoneal macrophages and plasma in hemorrhagic shock rats

作　　者：陈斌[1] 倪勇[1] 陈剑雄[2] 王成友[1] 

机构地区：[1]深圳市红十字会医院普外科,518035 [2]深圳市红十字会医院检验中心,518035

出　　处：《中华实验外科杂志》2001年第6期508-509,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　研究大鼠失血性休克前后血浆及腹腔巨噬细胞释放细胞因子的变化。方法　将 30只大鼠分为失血性休克组 ( 2 0只 )和对照组 ( 10只 ) ,观察大鼠休克前后血浆和腹腔巨噬细胞释放肿瘤坏死因子 (TNF) α、白细胞介素 (IL) 1β、IL 6和IL 8改变。 结果　失血性休克 90min后大鼠腹腔巨噬细胞释放TNF α、IL 1β、IL 6和IL 8显著减少 ( P <0 .0 1) ,而血浆中这些细胞因子水平明显升高 (P <0 .0 1)。结论　失血性休克对大鼠细胞免疫功能有抑制作用。Objective To study the change of cytoki ne s in the peritoneal macrophage supernatants and plasma in hemorrhagic shock rats .Methods Thirty rats were randomly divided into hemorrhagi c shock group (n=20) and control group (n=10).Tumor necrosis factor (TNF )-α,interleukin (IL)-1β,IL-6,IL-8 in peritoneal macrophage supernatants and plasma were measured before and after shock.Results TNF-α,IL-1β,IL-6 and IL-8 levels were sig nificantly decreased in peritoneal macrophage supernatants,but significantly in creased in plasma in hemorrhage shock rats.Conclusion Cell-mediated immunity is markedly depressed after hemorrhage shock.

关 键 词：巨噬细胞 细胞因子 大鼠 失血性休克 

分 类 号：R605.97[医药卫生—急诊医学]