参芍片中人参皂苷R_(g1),R_e和芍药苷含量的HPLC研究  

Determination of Content of Ginsenoside R_(g1) ,R_e, and Paeoniflorin from Shenshao Tablet by HPLC

作　　者：封士兰[1] 董钰明[2] 段生玉[2] 曲守伟[3] 李永民[1] 陈立仁[1] 

机构地区：[1]中国科学院兰州化物所,甘肃兰州73000 [2]兰州医学院药学系,甘肃兰州730000 [3]空军总医院,北京100000

出　　处：《中成药》2002年第1期25-27,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :高效液相色谱法测定参芍片中人参皂苷Rg1,Re 和芍药苷的含量。方法 :SymmetryC18柱。流动相 :乙腈 0 .2 %磷酸 (11∶89) ,流速 :0 .8mL/min ,柱温 :30°C ,检测波长 :2 0 3,2 30nm ,进样量 10 μL。 结果 :本法可测定人参皂苷Rg1,Re 和芍药苷的含量 ,其分别在 0 .96 0 μg～ 4.80 0 μg ;0 .912 μg～ 4.5 6 0 μg ;1.0 5 0 μg～5 .2 5 0 μg范围内与峰面积成线性关系。平均回收率分别为 10 5 .2 % ;98.5 % ;10 3.5 %。结论 :该方法简便、准确 。Objective:To determine the content of ginsenoside R g1 ,R e and Paeoniflorin by HPLC in Shenshao tablet.Methods: HPLC Symmetry C 18 column was used and CH 3CN H 3PO 4 (11∶89) as mobile phase. The flow rate was 0.8mL/min, the column temperature at 30 °C and the detective wavelength 203nm and 230nm. Results:The content of ginsenoside R g1 ,R e, and paeoniflorin can be determined by HPLC. The linear concentration ranges of these compound were 0.960μg～4.800μg, 0.912μg～4.560μg,1.050μg～ 5.250 μg. The average recoveries were 105.2%, 98.5% and 103.5%, respectively.Conclusion: This method is simple and accurate and can be used to determine the content of Shenshao Tablet.

关 键 词：人参皂苷RG1 芍药苷 参芍片 高效液相色谱法 人参皂苷RE 

分 类 号：R286.0[医药卫生—中药学]