抗菌肽MB_(39)基因导入‘皇家嘎啦’苹果及其四倍体植株的培育  

Regeneration of Tetraploid Plants with Cecropin MB_(39) Gene from'Royal Gala'Apple

作　　者：刘庆忠[1] 赵红军[1] 刘鹏[1] Mong Rengong FreddiA.Hammerschlag 

机构地区：[1]山东省果树研究所,泰安271000 [2]USDA/ARSHCRL [3]USDA/ARS,FruitLaboratory,Beltsville

出　　处：《园艺学报》2001年第5期392-398,共7页Acta Horticulturae Sinica

基　　金：山东省科委良种产业化工程资助项目 ;山东省人事厅资助项目 ;山东省农业科学院资助项目 (98 0 6 )

摘　　要：采用农杆菌介导法 ,将抗菌肽MB39基因转入‘皇家嘎啦’苹果 ,获得了 7个转基因株系。通过秋水仙素诱变染色体组工程育种技术 ,由转化体叶片获得了 2 0个四倍体植株。经PCR检测和SouthernBlotting杂交证明 ,抗菌肽MB39基因已经整合到皇家嘎啦苹果的染色体组中。转基因株系TR 1、TR 3提高了对火疫病的抗性。采用流式细胞技术 (FlowCytometry)As part of the program to develop transgenic Malus × Domestic. cv.'Royal Gala'tetraploid with improved traits,new germplasm of transgenic diploid and tetraploid plants with cecropin MB 39 gene were regenerated.Seven transgenic diploid plants were obtained from etiolated internodal explants by Agrobacterium tumefaciens mediated transformation using the plasmid binary vector pGV containing a chimeric gene cecropin MB 39 .The integration of the cecropin gene into apple genome was confirmed by PCR and Southern blotting analysis.It was proved that lines,TR 1 and TR 3,had increased the resistance to fireblight disease caused by Erwinia amylovora.Twenty tetraploid lines were produced by cocultivating leaf explants from wild type and transgenic diploid shoots with colchicine in apple regeneration medium.Flow cytometry was used for ploidy determination.The tetraploid plants were distinguishable from the diploid on morphological as well as cytogenetic grounds.Both the transgenic diploid and tetraploid plants are now being evaluated for growth development and disease resistance.

关 键 词：抗菌肽MB39 转基因 四倍体 秋水仙素 皇家嘎啦苹果 农杆菌介导 

分 类 号：S661.103.6[农业科学—果树学]