百合的花药培养研究  

Studies on Anther Culture of Lily

作　　者：褚云霞[1] 陈龙清[2] 黄燕文[1] 张永春[3] 

机构地区：[1]华中农业大学理学系,武汉430070 [2]华中农业大学林学系,武汉430070 [3]上海市农业科学院园艺研究所,上海201106

出　　处：《园艺学报》2001年第5期472-474,共3页Acta Horticulturae Sinica

基　　金：武汉市科技攻关项目 (992 0 0 2 0 5 0G)

摘　　要：对百合花药培养的影响因素研究显示 ,小孢子最适培养时期为单核期 ;愈伤组织诱导率最高可达 4 2 .16 % ,最佳激素组合为 2 ,4 D 3.0mg·L- 1+KT 3.0mg·L- 1;低温预处理作用不大 ;早分化出的花蕾中的小孢子更易诱导产生愈伤组织 ;分化培养基以改良MS +NAA(2 .0、 4 .0mg·L- 1)为宜 ;花粉植株中单倍体 (n =12 )约 2 5 % ,二倍体 (2n =2 4 )约 75 %。The following experimental results have been obtained:Compared with other stage,microspores at uninucleate stage were more suitable for culture.Calli were induced from anthers of lily placed on modified MS media supplemented with different concentrations of 2,4 dichlorophenoxyacetic acid(2,4 D)and Kinetin(KT)according to homogeneous design.The cold pretreatment(4℃,2 days)couldn't obviously raise the callus induction frequency in anther culture;The pollen calli were transferred to modified MS media with different concentrations of naphthaleneacetic acid(NAA).The results indicated that modified MS media with 2.0-4.0mg·L -1 NAA were optimal for the differentiation of pollen callus.Cytological examination of mitotic root tip cells from green plants showed that about 25% of them were haploid and others were diploid.

关 键 词：百合 组织培养 花药培养 影响因素 培养基 培养条件 

分 类 号：S682.290.3[农业科学—观赏园艺]