肝细胞生成素快速诱导肝再生相关基因mRNA的表达  

Rapid induction of mRNAs for liver regeneration genes by Hepatopoietin

作　　者：王阁[1] 冷恩仁[1] 胡辂[1] 王军[1] 房殿春[2] 杨晓明[3] 张咏[3] 贺福初[3] 

机构地区：[1]中国人民解放军第三军医大学大坪医院野战外科研究所消化内科,重庆市400042 [2]中国人民解放军第三军医大学西南医院消化内科,重庆市400038 [3]军事医学科学院放射医学所,北京市100850

出　　处：《世界华人消化杂志》2002年第2期169-172,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.29900073;39870005~~

摘　　要：目的:观察人重组肝细胞生成素(rhHPO)迅速诱导瞬时早期反应基因表达情况.方法:利用PT-PCR方法观察HPO刺激原代培养大鼠肝细胞24h内选择性肝再生相关基因mRNA表达情况.结果:HPO在原代培养大鼠肝细胞体系中可迅速诱导瞬时早期反应基因c-fos,LRF-1d的表达,对PC3和Tec基因也有诱导表达的作用,高峰出现在刺激后的0.5-2h,表达量增加3-5倍.结论:HPO可迅速诱导瞬时早期反应基因表达,可能是HPO促肝细胞增生的分子作用机制,首次报道PC3和Tec基因是一种与肝再生调控密切相关的早期反应基因.AIM:To investgate recombinant human Hepatopoietin (rhHPO) rap idly induces the expression of immediate early gene. METHODS:We investigated the different gene mRNA expression relat ed liver regeneration within 24 h by PT-PCR in primary cultured hepatocytes sys tem. RESULTS:HPO can rapidly induce the expression of immediate early gene (c-fos, LRF-1, PC3 and Tec, etc.) in primarily cultured rat hepatocytes. The levels of c-fos and LRF-1 mRNA ,which are also known to be induced during the early hours of liver regeneration, were increased after treatment of isolat ed hepatocytes with rhHPO. Addition of HPO resulted in rapid and transient induc tion of PC3 and Tec mRNA with a peak 0.5-2 h(3-5fold). CONCLUSION:rhHPO can rapidly induce the expression of im mediate early gene, which might be one of HPO molecular mechanism on stimulatin g hepatocyte proliferation. This is the first report that PC3 gene and Tec gene are immediate early gene related to liver regeneration.

关 键 词：肝再生 肝细胞生成素 PT-PCR方法 C-MYC基因 

分 类 号：R346[医药卫生—基础医学]