白介素-10抑制肝星状细胞细胞间黏附分子-1的表达  

Interleukin-10 inhibit intercellular adhesion molecule-1 expression in isolated rat hepatic stellate cells

作　　者：翁山耕[1] 冷希圣[1] 魏玉华[1] 彭吉润[1] 杜如昱[1] 

机构地区：[1]北京大学人民医院外科,100044

出　　处：《中华消化杂志》2001年第8期458-460,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目 (39970 72 2 )

摘　　要：目的　探讨白介素 10 (IL 10 )对肝星状细胞 (HSC)细胞间黏附分子 1(ICAM 1)表达的影响。方法　采用肝脏离体胶原酶灌注消化及密度梯度离心的方法来分离培养HSC ,传代后的HSC随机分为 4组 :对照组 (A组 )、肿瘤坏死因子α(TNFα) 10 0U/ml组 (B组 )、TNFα 10 0U/ml+IL 10 2ng/ml组 (C组 )及TNFα 10 0U/ml +IL 10 2 0ng/ml组 (D组 )。加药后 2 4h ,采用细胞酶联免疫吸附分析(ELISA)和半定量逆转录聚合酶链反应 (RT PCR)等方法 ,检测HSCICAM 1蛋白及mRNA表达。结果　B组的ICAM 1蛋白和mRNA表达量分别为 1.40 0± 0 .0 77和 1.30 1± 0 .0 95 ,明显高于A组的 0 .5 5 9± 0 .0 71(P <0 .0 0 1)和 0 .6 6 6± 0 .0 2 3(P <0 .0 0 1) ;C组和D组的ICAM 1蛋白表达量分别为 1.0 17±0 .0 6 6和 0 .919± 0 .0 39,mRNA表达量分别为 1.116± 0 .0 17和 0 .979± 0 .0 6 7,均低于B组的ICAM 1蛋白和mRNA表达 (P均 <0 .0 5 )。结论　IL 10通过抑制HSCICAM 1表达 ,在抑制肝脏炎症。Objective To investigate the effects of interleukin 10(IL 10) on the intercellular adhesion molecule 1(ICAM 1) expression of rat hepatic stellate cells. Method Hepatic stellate cells were isolated and purified from rat liver by collagenase Ⅳ perfusion and density gradient centrifugation with Nycodenz. Passaged hepatic stellate cells were divided randomly into four groups: control group (A group), TNFα 100 U/ml group (B group), TNFα 100 U/ml plus IL 10 2 ng/ml group (C group). TNFα 100 U/ml plus IL 10 20 ng/ml group (D group). 24 h after incubation expression of ICAM 1 protein and mRNA in hepatic stellate cells were detected by using cell ELISA and semi quantitative RT PCR method. Results The yield rate of hepatic stellate cells was 2.5×10 7 per rat. The cell viability was above 98%. The purity of hepatic stellate cells was more then 92%. Expression of ICAM 1 protein (1.400 ±0.077) and mRNA(1.301±0.095) in B group were significantly higher than that in A group [0.559±0.071 ( P <0.001) and 0.666±0.023 ( P < 0.001) ] respectively. Expression of ICAM 1 protein in C group and D group were 1.017±0.066 and 0.919 ± 0.039, respectively, and expression of ICAM 1 mRNA in C group and D group were 1.116±0.017 and 0.979 ±0.067 respectively, which were lower than the expression of ICAM 1 protein ( P < 0.05) and mRNA( P < 0.05) in B group. Conclusion IL 10 can inhibit ICAM 1 expression in rat hepatic stellate cells, which might play a protective role against liver inflammation and fibrosis.

关 键 词：肝星状细胞 白介素-10 细胞间黏附因子-1 抑制 肝炎 肝纤维化 

分 类 号：R657.3[医药卫生—外科学]