高效液相色谱法测定环氯滴眼液的含量  

Determination of Cyclocytidine and Chlorampnenicol Eye Drops by HPLC

作　　者：白林[1] 吕小川[1] 魏萍[2] 

机构地区：[1]中国人民解放军总医院药材处,北京100853 [2]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071

出　　处：《解放军药学学报》2001年第5期280-281,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　测定环氯滴眼液中环孢苷、氯霉素的含量。方法　高效液相色谱法 ,采用YWG -C18(10 μm ,4.6mm× 15 0mm)色谱柱 ,以 0 .0 1mol·L-1磷酸二氢钠 -甲醇 -乙氰 (30 0∶16 0∶40 ,三乙胺 0 .5ml,磷酸调pH3 .0 )为流动相 ,检测波长为 2 6 3nm ,内标法峰高定量。结果　可同时测定 2组分的含量 ;环孢苷在 5～ 2 5 μg·ml-1、氯霉素在 10～ 5 0 μg·ml-1范围内 ,峰高比与其浓度均呈良好的线性关系 ;平均回收率为 99.0 % ;10 0 .2 5 % ;RSD分别为 1.5 5 % ;0 .94%。结论　方法可靠 ,数据准确 ,灵敏度高 ,重复性好。Aim To establish a HPLC assay for the detemination of Cyclocytidine and Chloramphenicol Eye Drops Methods: In the method YWG C 18 (10μm, 4.6mm×150mm) column as the stationary phase and 0.01mol·L 1 Sodium dihydrogen phosphate methanol acetinitrile (300∶160∶40) as mobil phase, the determination wavelength of 263nm. Results: The separation and determination of the two constituents could be accomplished simultaneously. The calibration curves were linear in the range of 5～25 μg·ml 1 for Chloramphenicol. The average recoveries of Cyclocytidine, Chloramphenicol were 99.0%(RSD=1.55%) and 100.25% (RSD=0.94%) respectively. Conclusion: This method was found to be simple, quick and accurate for simultaneous determination of the two components in Cyclocytidine and Chloramphenicol Eye Drops.

关 键 词：高效液相色谱法 环孢苷 氯霉素 含量测定 

分 类 号：R988.1[医药卫生—药品] R927.2[医药卫生—药学]