颅脑损伤后急性期脑脊液中一氧化氮及其合成酶的动态观察  

Continua observation of nitric oxide and nitric oxide synthase in cerebrospinal fluid during acute period of brain injury

作　　者：张永和[1] 赵宁[2] 宋祖军[1] 

机构地区：[1]第四军医大学西京医院急诊科,西安710032 [2]第四军医大学生物技术中心

出　　处：《中国急救医学》2001年第12期701-702,共2页Chinese Journal of Critical Care Medicine

摘　　要：目的　检测一氧化氮 (NO)及其合成酶 (NOS)在颅脑损伤后急性期脑脊液中的变化 ,探讨其临床意义。方法　采用检测NO的中间代谢产物亚硝酸盐来反映NO及放射强度测定法测定NOS活性 ,分别测定颅脑损伤后急性期脑脊液中NO和NOS变化 ,并行健康对照。结果　颅脑损伤后NO及NOS脑脊液中浓度第 1天即增高 ,NO第 3天达到高峰后开始下降 ,至第 10天仍高于正常对照组 ;NOS于损伤后第 1天开始升高 ,至第 10天仍高于正常对照组。结论　NO和NOS参与了颅脑损伤的病理生理过程 ,动态观察提示NO可能和颅脑损伤病情程度有关。Objective To investigate the change of nitric oxide (NO) and nitric oxide synthase(NOS) in cerebrospinal fluid (CSF) of patients during acute period of brain injury, and to explore the clinical significance. Method NO concentration in CSF of patients was determined by the methods of measuring of its metabolic product of nitrite, nitric oxide synthase was observed simultaneously. Results Both concentration of NO and NOS was increased at day 1 post-injury. After getting the highest levels at day 3 post-injury, NO began to drop gradually, and both NO and NOS was still at higher levels than that of control group at day 10 post-injury. Conclusion NO and NOS were involved in the pathological process of brain injury and might have relevant with the severe of injury.

关 键 词：颅脑损伤 脑脊液 一氧化氮 一氧化氮合成酶 

分 类 号：R651.15[医药卫生—外科学] R446.14[医药卫生—临床医学]