兰州百合和野百合组织培养及快速繁殖研究  

Study on tissue culture and clonal propagation of Lilium davidii var.Unicolor(Hoog)Cotton and Lilium brownii F.E.Brown ex Miellez

作　　者：王刚[1] 杜捷[1] 李桂英[1] 梁万福[1] 幸亨泰[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2002年第1期69-71,共3页Journal of Northwest Normal University(Natural Science)

基　　金：西北师范大学科技创新工程资助项目 (NWNU KJCXGC 0 2 )

摘　　要：以兰州百合 (LiliumdavidiiVar.Unicolor (Hoog)Cotton)和野百合 (LiliumbrowniiF .E .BrownexMiellez)鳞茎及叶片为外植体获得再生植株 ,并建立起快速无性繁殖系 .兰州百合鳞茎的最佳诱导培养基为MS +0 6～ 1 0mg/LBA +0 2mg/LNAA ;芽增殖培养基选MS +0 5～ 0 6mg/LBA +0 1mg/LNAA ;生根培养基为 12 MS +0 2mg/LIAA +0 1%活性炭 .野百合鳞茎及叶的最佳诱导培养基分别为MS +0 1～ 0 5mg/LBA +0 1～ 0 2mg/LNAA或 0 5mg/LIAA和MS +0 7mg/LBA +0 0 7mg/LNAA ;鳞茎诱导的芽的增殖培养基为MS +0 2～ 0 4mg/LBA +0 1mg/LNAA ;生根培养基为 12 MS +0 15mg/LNAA +0The clonal propagation of Lilium davidii var.Unicolor(Hoog)Cotton and Lilium brownii F.E.Brown ex Miellez is developed through culture of scales and leaves in Vitro.The best induction medium of Lilium davidii var.Unicolor(Hoog)Cotton scales is MS+0.6～1.0?mg/L BA+0.2?mg/L NAA.Proliferation medium is MS+0.5～0.6?mg/L BA+0.1?mg/L NAA.Induction of root is better for shoots in 12MS+0.2?mg/L IAA+0.1% active charcoal.Induction medium of Lilium brownii F.E.Brown ex Miellez shoots is MS+0.1～0.5?mg/L BA+0.1～0.2?mg/L NAA(0.5?mg/L IAA)and MS+0.7?mg/L BA+0.07?mg/L NAA.Proliferation medium of scales is MS+0.2～0.4?mg/L BA+0.1?mg/L NAA.The better medium for roots induction is 12?MS+0.15?mg/L IAA+0.1% active charcoal.

关 键 词：兰州百合 野百合 组织培养 无性繁殖 栽培技术 

分 类 号：S644.103.6[农业科学—蔬菜学] S682.29[农业科学—园艺学]