东北红豆杉愈伤组织培养研究  

Study on Callus Culture of Taxus Cuspidata

作　　者：王森林[1] 胡风庆[1] 

机构地区：[1]辽宁大学生命科学系,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2002年第1期75-77,共3页Journal of Liaoning University：Natural Sciences Edition

摘　　要：以东北红豆杉幼茎为培养材料诱导出愈伤组织 ,并分析了不同的激素组合、糖浓度、培养条件对愈伤组织诱导的影响 .结果表明 ,NAA激素组合比 2 ,4—D组合更有利于愈伤组织的诱导 ,在 2 5℃、2 %蔗糖、0 .7%琼脂、每天光照 1 2h条件下愈伤组织诱导率达1 0 0 % .Calli were induced from young stem of Taxus cuspidata. The effects of hormone, sugar concentration and culture condition on the inductivity were studied. The results indicated that NAA combination was more effective on inducing callus than 2,4-D combination. Under condition of 2%sugar concentration, 0.7%agar concentration, 25℃ and illumination 12h/day, the rate of callus inductivity was 100%.

关 键 词：东北红豆杉 愈伤组织 诱导率 紫杉醇 抗癌药物 组织培养 

分 类 号：Q949.661[生物学—植物学] R979.1[医药卫生—药品]