大豆甾醇侧链的生物降解  

Microbial Transformation of Soybean Sterol

作　　者：车成彬[1] 刘景春 吴宝华[1] 

机构地区：[1]哈尔滨理工大学应用科学学院,黑龙江哈尔滨150080 [2]哈尔滨食品研究所,黑龙江哈尔滨150000

出　　处：《哈尔滨理工大学学报》2002年第1期93-95,共3页Journal of Harbin University of Science and Technology

摘　　要：通过采用诺卡氏菌和分枝杆菌对大豆甾醇侧链降解能力的测定，筛选出一株分枝杆菌Ｍ６６．发醇过程中加入甾核降解抑制剂，可以得到甾体侧链限制性降解产物雄甾—１，４—二烯—３，１７—二酮（ＡＤＤ）．实验表明：分枝杆菌Ｍ６６最佳实验室小试发酵条件的筛选结果，底物转化７２ｈ，抑制剂为 ＣｏＳＯ４·７Ｈ２Ｏ，其质量分数为 ０．０３％时，大豆甾醇侧链的降解转化率达 ６０％以上，ＡＤＤ转化率达２５％左右．The ability of microorganisms(Nocardia and Mycobacterium) is studied to degrade the soybean sterol; A species has been obtained, which is Mycobacterium M66. If we get the inhibitor to degrade the sterol nuclear into the leaven solution, we can accumulate inhibition degraded products of sterol side-chains, it is androst-1.4-diene-3.17-dione(ADD). The result is reported of the best condition small experiment the transformation time ws 72h, the transformation rate of soybean sterol was 60% and the yield of ADD was about 25% when Mycobacterium M66 was used and in the presence of 0.03% CoSO4·7H2O as an inhibitor.

关 键 词：甾醇侧链 生物降解 抑制剂 大豆 诺卡氏菌 甾体药物 

分 类 号：TQ464[化学工程—制药化工] TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]