ApoE基因敲除鼠12周游泳运动前后纤溶激活功能的改变  

Plasminogen Activation Changes in ApoE Gene Deficiency Mice after 12-week Swimming

作　　者：李晖[1] 李可基[1] 张宝慧[1] 拾景达[1] 

机构地区：[1]北京大学第三医院运动医学研究所,北京100083

出　　处：《中国运动医学杂志》2002年第1期15-18,共4页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金资助项目No.39770813

摘　　要：目的：探讨ＡｐｏＥ在长期有氧运动中影响纤溶激活作用中的有关机制。方法：以ＡｐｏＥ基因敲除（ＡｐｏＥ－／－）鼠为实验组、以相同遗传背景的Ｃ５７ＢＬ／６Ｊ鼠为对照组建立运动模型。结果：１２周游泳后，对照组鼠ＴＧ（０．９６±０．３０ｖｓ０．５４±０．０７０ｍｍｏｌ／Ｌ，Ｐ＜０．０１）和ＰＡＩ（０．９２±０．０７ｖｓ０．８０±０．０９ＡＵ／ｍｌ，Ｐ＜０．０５）降低，ｔＰＡ升高（０．６０±０．１２ｖｓ１．０４±０．２３ＩＵ／ｍｌ，Ｐ＜０．０１）；而ＡｐｏＥ－／－鼠的ＴＧ（１．２８±０．３４ｖｓ１．２９±０．３２ｍｍｏｌ／Ｌ，Ｐ＞０．０５）和ＰＡＩ（０．７４±０．１０ｖｓ０．７９±０．０４ＡＵ／ｍｌ，Ｐ＞０．０５）均没有显著变化，ｔＰＡ（０．７１±０．１５ｖｓ０．９７±０．１７ＩＵ／ｍｌ，Ｐ＜０．０１）升高。结论：长期有氧运动使对照小鼠纤溶激活作用明显改善，其机制与ＴＧ和含ＡｐｏＥ脂蛋白水平降低导致ＬＲＰ清除ｔＰＡ－ＰＡＩ作用加强、ＰＡＩ抑制作用减弱及ｔＰＡ合成水平增加有关。Ａｐ ｏＥ－／－鼠运动后，ＰＡＩ水平稳定而ｔＰＡ活性增加，提示ｔＰＡ合成作用增强。Objective To approach ApoE relevant mechanism in fibrinolytic changes induced by chronic aerobic exercise. Methods ApoE gene knockout (ApoE-/-) mices were used for the experiment and the homogeneous mice of C57BL/6J as the control. Results After swimming for 12 weeks, in control group, TG(0.96±0.30 vs 0.54±0.070 mmol/L,P < 0.01)and PAI(0.92±0.07 vs 0.80±0.09 AU/ml,P < 0.05) decreased and tPA (0.60±0.12 vs 1.04±0.23 IU/ml,P < 0.01) increased significantly; in experimental group, TG (1.28±0.34 vs 1.29±0.32 mmol/L , P > 0.05 )and PAI (0.74±0.10 vs 0.79±0.04 AU/ml,P > 0.05)changed insignificantly and tPA (0.71±0.15 vs 0.97±0.17 IU/m1,P < 0.01 )increased significantly. Conclusion Chronic aerobic exercise increased fibrinolytic activity in control group, the mechanism in which is considered to corelate with faster LRP clearing tPA -PAI complexes induced by reduction of TG rich and ApoE contained lipoprotein, and higher levels of PAl inhibition and tPA production. Increased activity of tPA in ApoE-/-mice after exercise was accompanied with an unchanged activity of PAI, implying more tPA production.

关 键 词：ApoE基因敲除鼠 纤溶激活作用 组织纤溶激活因子 纤溶激活抑制因子 低密度脂 蛋白受体相关蛋白 游泳 

分 类 号：R87[医药卫生—运动医学]