栝楼中α-菠菜甾醇的含量测定  

DETERMINATION OF α-SPINASTEROL IN TRICHOSANTHES KIRILOWII MAXIM

作　　者：刘拥军[1] 时岩鹏[1] 姚庆强[1] 栾杨[1] 

机构地区：[1]山东省医学科学院药物研究所,山东济南250062

出　　处：《华西药学杂志》2002年第1期58-59,共2页West China Journal of Pharmaceutical Sciences

基　　金：山东省自然科学基金资助 (项目编号 :Q99C0 8)

摘　　要：目的　测定中药栝楼中α 菠菜甾醇的含量 ,建立栝楼生药质量的检测方法。方法　栝楼以氢氧化钠甲醇液回流提取 ,以石油醚 -乙酸乙酯 -甲醇 (8∶2∶1)为展开剂 ,进行双波长薄层扫描 ,检测波长 4 0 0nm ,参比波长6 0 0nm。结果　栝楼中α 菠菜甾醇的含量为 0 .0 171% ,线性范围 1.0～ 2 .0 μg ,r =0 .990 0 ,回收率 93.7% ,RSD =2 .86 % (n =5 )。结论　该方法灵敏性高 ,稳定性和重复性较好 ,可作为栝楼生药的检测方法。OBJECTIVE To control the quality of Trichosanthes kirilowii Maxim by determining the quantity of α spinasterol. METHODS Trichosanthes kirilowii Maxim was extracted by methanol,then determined by TLCS.The developing solution was petroleum-ethyl acetate-formic acid(8∶2∶1).The detection wavelength was 400 nm and reference wavelength was 600 nm.RESULTS The content of α spinasterol in Trichosanthes kirilowii Maxim was 0.0171%. The linear relationship was obtained in the range of 1.0-2.0 μg(r=0.9900),the recovery was 93.7%,RSD=2.86%(n=5).CONCLUSION This method is sensitive, stability and reproductive. It can be used to control the quality of Trichosanthes kirilowii Maxim.

关 键 词：薄层扫描法 栝楼 α-菠菜甾醇 含量测定 中药材 

分 类 号：R284.1[医药卫生—中药学]