PCR检验技术在性病临床标本实验诊断中应用价值的研究  

Evaluation of the Application of PCR in Laboratory Diagnosis for Sexually Transmitted Diseases

作　　者：叶顺章[1] 王千秋[1] 王荷英[1] 李珊山[2] 张树文[1] 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所全国性病麻风病控制中心,江苏南京210042 [2]白求恩医科大学第一临床学院,吉林长春130021

出　　处：《中国皮肤性病学杂志》2002年第1期6-8,共3页The Chinese Journal of Dermatovenereology

基　　金：世界银行卫V贷款资助

摘　　要：目的　评价聚合酶链反应 (PCR)检测技术在性病临床诊断中的应用价值。方法　比较PCR与传统检测手段对淋病和非淋菌性尿道炎病原体的检测结果。淋球菌以加多粘菌素的GC血液琼脂分离 ;衣原体用McCoy细胞培养 ;支原体用高效支原体培养基培养。PCR试验用基因释放剂作前处理 ,以 1.5 %琼脂糖凝胶电泳观察结果。结果　用PCR法检查淋病、衣原体和支原体感染的临床标本其敏感性和特异性均比传统的培养法高 ,作PCR试验的标本取材要求不如作培养那样严格 ,但作PCR应使用正规的试剂盒、规范的操作和严格的防污染措施 ,否则极易引起交叉污染。结论　PCR检验技术在性病诊断中具有巨大的应用潜力 ,对一些培养困难的病原菌 ,可在规范操作和标准化试剂盒的基础上逐步加以使用。PCR检测不宜用作判愈。Objective To evaluate the significance of the application of PCR in laboratory diagnosis for sexually transmitted diseases.Methods To compare the results of PCR and traditional methods in detecting the pathogens of gonorrhea and non gonococcal urethritis(NGU).The laboratory techniques were GC agar supplemented by blood and polymyxin B for NG culture,McCoy cell culture for CT,and high effective liquid culture medium for UU.Clinical samples were also treated by gene releaser for PCR assay and the amplicons were visualized by agar electrophoresis.Results The sensitivities and specificities of PCR were higher than those of traditional culture methods for the testing of NG,CT and UU.The sampling for PCR was not as strict as that for culture methods.However,it was important to use approved PCR kit,observe standardized procedures,and take measures against contamination when PCR assay was conducted.Otherwise cross contamination might happen.Conclusion PCR has potential application value for the laboratory diagnosis of sexually transmissible pathogens.This technique could be applied for the detection of pathogens which are difficult to be cultured,provided standardized procedures and approved kit are taken into consideration.PCRcan not be used for test of cure.

关 键 词：聚合酶链反应 淋球菌 衣原体 支原体 诊断 

分 类 号：R759.04[医药卫生—皮肤病学与性病学]