多种神经组织修复成鼠脊髓损伤的实验研究  

Intraspinal transplantation of different nervous tissue to repair spinal cord injury:a study by comparison in adult rats

作　　者：郭庆山[1] 王爱民[1] 蒋祖言[1] 孙红振[1] 杜全印[1] 

机构地区：[1]第三军医大学大坪医院显微骨科,重庆400042

出　　处：《创伤外科杂志》2002年第1期20-23,共4页Journal of Traumatic Surgery

摘　　要：目的　探讨不同神经组织移植修复成鼠急性脊髓损伤 (SCI)的能力。方法　成鼠脊髓损伤后 ,分别移植游离正中神经 (FPN组 )、带血管蒂正中神经 (VPN组 )、孕 1 4天胚胎脊髓 (FSC组 )、游离正中神经加胚胎脊髓 (P +F组 )、带血管蒂正中神经加胚胎脊髓 (V +F组 )。术后 8周行神经解剖及电生理检查。结果V +F组再生轴突和存活雪旺氏细胞数目、胚胎脊髓体积增长速度和神经元密度显著高于对照组 (P <0 .0 1 ) ,细胞分化较好 ,突触较成熟。体感诱发电位 (SEP)的P1 、N1 波潜伏期显著缩短 (P <0 .0 1 )。结论　带血管蒂周围神经与胚胎脊髓联合移植 ,在解剖和电生理上均优于其他组织 。Objective To observe the effects of different nervous tissue graft for repair of the spinal cord injury in adult rats.?Methods A 5 mm cord defect of the left lateral column was made at T 1-3 vertebral level in adult rats, the defect was grafted randomly using free peripheral nerve (Group FPN), vascularized peripheral nerve (Group VPN), embryonic day 14 fetal spinal cord (Group FSC), free peripheral nerve and fetal spinal cord (Group P+F), vascularized peripheral nerve and fetal spinal cord (Group V+F) respectively.Eight weeks after surgery, the survival, differentiation of the grafts, and the ability in repairing host spinal cord were assessed by histological analysis and somatosensory evoked potential (SEP) studies.?Results In Group V+F, peripheral nerve segments were well innervated, plenty of Schwann cells also survived and proliferated. The fetal implant was intimately fused with the host CNS parenchyma, the volume, nerve fiber and neurons density were significantly higher than groups FSC and P+F (P<0.01). SEP assessments showed that the latency of P 1 , N 1 wave was significantly decreased in this group (P<0.01).?Conclusion The VPN FSC co graft group has an advantage over the control groups in histological and SEP, this combination could prompt the development and differentiation of fetal implants, support the regeneration of central injured axons to certain degrees.

关 键 词：周围神经 胚胎脊髓 组织修复 脊髓损伤 

分 类 号：R651.2[医药卫生—外科学]