柞蚕丝素基因5′端部分序列的克隆及结构分析  

Cloning and Structure Analysis on 5′ Flanking Sequence of Fibroin Gene of Chinese Oak Silkworm

作　　者：李文利[1] 金礼吉[1] 范琦[1] 安利佳[1] 

机构地区：[1]大连理工大学生物工程系,大连116023

出　　处：《中国农业科学》2002年第2期218-221,共4页Scientia Agricultura Sinica

基　　金：辽宁省自然科学基金资助项目 (4 180 64 )

摘　　要：利用PCR技术从中国柞蚕品种 74 1中扩增并克隆出柞蚕丝素基因 5′端部分片段 ,它由CAAT盒、TATA盒 (Hognessbox)、primtranscript、丝素蛋白的起始密码子ATG和部分结构基因及内含子组成。通过与天蚕丝素基因的 5′端序列进行比较 ,发现 12 4～ 5 0 6bp、796～ 1194bp、12 16～ 12 98bp同源性高 ,同源率分别为91.6 %、95 %、95 %。将柞蚕丝素基因 5′端的CAAT盒、TATA盒、primtranscript序列与KikuchiY所测家蚕、HuiCC所测家蚕及家蚕P2 5蛋白、天蚕丝素基因相应序列进行比较 ,发现柞蚕与天蚕相应序列的同源率远高于家蚕。从序列中可以看出 :若转录起始点第一个核苷酸标为 +1,则TATA盒位于 - 2 5处 ;CAAT盒位于 - 70处。The 5′ flanking fragment of fibroin gene of Chinese Oak Silkworm(Antheraea pernyi) was amplified through PCR. It consists of CAAT box, TATA box(Hogness box), prim transcript, start code ATG, part of structural gene and first intron. Compared with Japanese Oak silkworm(Antheraea yamamai), three high homology regions have been observed in the sequence of the 5′ flanking(nt.86～479bp, nt.769～1167bp and nt.1189～1303bp),with the similarity of 91.6%, 95% and 95%,respectively. As for CAAT box, TATA box and prim transcript, the homology between Chinese Oak Silkworm and Japanese Oak silkworm is higher than that between Chinese Oak silkworm and Bombyx mori.The TATA box locate at the upstream -25bp and CAAT box at -70bp from the prim transcript, which is similar as the character of eukarytor promoter.

关 键 词：柞蚕丝 丝素基因 柞蚕 序列分析 启动子 5′端序列 结构 克隆 

分 类 号：S885.1[农业科学—特种经济动物饲养]