GDNF对海仁酸损伤体外培养新生大鼠背根神经节神经元的影响  

The Effects of GDNF Against Kainic Acid Excitotoxic Injury on Neonatal Rat Doral Root Ganglion Neurons in Vitro

作　　者：杨浩[1] 王春婷[1] 许汉鹏[1] 鞠躬[1] 

机构地区：[1]第四军医大学神经科学研究所细胞生物学研究室,陕西西安710032

出　　处：《动物医学进展》2002年第1期42-45,共4页Progress In Veterinary Medicine

摘　　要：Ｎ１无血清培养基原代分离培养新生大鼠背根神经节（　ｄｏｒｓａｌ　ｒｏｏｔ　ｇａｎｇｌｉｏｎ　ＤＲＧ）神经元，用兴奋性毒素　海仁酸（ｋａｉｎｉｃ　ａｃｉｄ　ＫＡ）损伤体外培养细胞后，加入胶质源性神经营养因子（ｇｌｉａｌ－ｄｅｒｉｖｅｄ　ｎｅｕｒｏｔｒｏｐｈｉｃ　ｆａｃｔｏｒ，　ＧＤＮＦ）作用，最后通过ＭＴＴ法检测、细胞总蛋白测定、胎盘兰染色计数、形态学观察等方法研究分析ＧＤＮＦ对兴奋性毒素海仁酸损伤后大鼠ＤＲＧ神经元的　活性、存活及突起生长的影响。结果表明：ＧＤＮＦ对体外正常生长的ＤＲＧ神经元的存活、活性　及突起生长没有明显的促进作用，而在海仁酸损伤后，ＧＤＮＦ对其存活和活性有明显的促进作用，但对突起生长没有明显的影响。：The purpose of this study is explored different neur otrophic effects of glial-derived neurotrophic factor (GDNF) on survival ,viabi lity and neurite outgrowth of injured DRG by KA-induced,so primarily cultured DRG neurons were dissociated from neonatal rats in N1 serum-free medium,inju rious model was set up by the addition of KA into the cultures in vitro .The via bility, survival , and outgrowth of neurite was determined and assessed by MTT a ssays, synthesis of total protein, trypan-blue staining cells count and morphol o gical observation,respectively , the results indicated that GDNF had no remark able effects on the viability ,survival and neurite length of DRG neurons under normal condition in vitro , whereas it significantly promoted viability and surv ival of DRG neurons except outgrowth of the neurites after KA -induced injury.

关 键 词：GDNF 背根神经节 海仁藻酸 兴奋性毒素 细胞培养 胶质源性神经营养因子 神经元 

分 类 号：R338.1[医药卫生—人体生理学] Q421[医药卫生—基础医学]