IL-2基因修饰对树突状细胞的生物学特征和功能的影响  

The effect of IL-2 gene modification on the biologic character and function in den-dritic ceIls

作　　者：曹雪涛[1] 张明徽[1] 章卫平[1] 刘海军[1] 孙黎飞 

机构地区：[1]第二军医大学免疫学教研室,上海200433 [2]济南军区肿瘤治疗中心淄博148医院肿瘤科工作

出　　处：《中国免疫学杂志》2002年第1期5-8,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金重点资助项目(39730420)

摘　　要：目的:观察白细胞介素2(IL-2)基因修饰对树突状细胞(DC)的生物学特征和功能的影响,探讨用IL-2基因修饰DC,增强DC介导特异性抗肿瘤免疫的机制。方法:IL－2基因修饰小鼠骨髓来源的DC后,用扫描电镜观察其表面形态的变化,FACS分析IL-2基因修饰对DC表面免疫分子表达的影响,RT-PCR方法检测DC中 IFN-γ mRNA表达。用3H-TdR掺入法检测IL-2基因修饰后,DC对同种异体T淋巴细胞的刺激作用和对肿瘤抗原的特异性提呈功能。结果:经IL-2基因修饰后,DC表面的伪足增多、变长;其表面与抗原提呈相关的免疫分子Ia、B7-1、B7-2和CD40的表达明显上调;il-2基因修饰的DC(DC-IL-2)中表达IFN-γ mRNA;CD-IL-2不但对同种异体T淋巴细胞有较强的促增殖作用,而且对肿瘤抗原的特异性提呈功能亦明显增强。结论:IL-2基因修饰DC,能促进DC的发育,上调DC表面与抗原提呈相关的免疫分子,增强了DC的生物活性。To observe the effect of IL-2 gene modification on the character of biology and function in dendritic cells( DC) and to investigate the immune mechanism of specific anti-tumor of IL-2 gene modification in DC. Methods: DCs were prepared from mouse bone marrow and genetically modified by IL-2 adenovirus. Then observe the changes of DC morphology by scanning electro-microscopy, analyzed molecules on DC by FACS, examined the expression of IFN-γ mRNA in DC by RT-PCR.The stimulatory capacity of DC to T cells detected by MLR their capacity of antigen present were measured by 3H-TdR mix into assay. Results: After IL-2 gene modification, the morphology of DC was changed, its pseudopod was more and longer. The expression of Ia, B7-1, B7-2 and CD40 molecules was more on DC surface. The IFN-7 mRNA was expressed in the DC-EL-2 and DC-rhIL-2.DC-IL-2 could stimulate allogeneic T cells more potently and IL-2 gene-modified DC could induce more potent antigen-specific autogeneic CTL. Cooclusioo: IL-2 genetic modification can promote DC growth and up regulate their expression of membrane immune molecules that are relevant for antigen presentation of DC,and enhanced the biologic activity of the DC.

关 键 词：白细胞介素2 树突状细胞 基因修饰 生长发育 抗原提呈 

分 类 号：R392.1[医药卫生—免疫学]