免疫层析试验与镜检法、聚合酶链反应诊断疟疾的比较  

Comparison of the immunochromatographic malaria P. f/P. v test with microscopic examination and PCR for diagnosis of malaria

作　　者：吴英松[1] 李明[1] 毕惠祥[1] 董文其[1] 李英杰[1] 

机构地区：[1]第一军医大学热带病研究室,广州510515

出　　处：《中华检验医学杂志》2002年第1期29-31,共3页Chinese Journal of Laboratory Medicine

基　　金：全军医药卫生科研基金课题 ( 96L0 34)

摘　　要：目的　评价免疫层析试验 (ICT)诊断疟疾的临床应用价值。方法　以镜检法和聚合酶链反应 (PCR)为对照 ,同时用ICT检测 89份门诊可疑疟疾感染患者的血标本。结果　ICT与镜检法和PCR比较 ,阳性检出符合率分别为 93 .4 %和 86.4 % ;敏感性分别为 90 .16%和 83 .3 3 % ;特异性分别为92 .86%和 91.3 0 %。当虫体密度 >10 0个 / μl时 ,ICT的敏感性为 10 0 % ,虫体密度 <10 0个 / μl时 ,敏感性为 72 .73 % ,虫体密度 <5 0个 / μl时 ,敏感性降低到 66.67%。 结论　ICT适用于疟疾检测的初筛 。Objective To evaluate the applicability of immunochromatographic test (ICT) in detecting malaria. Methods With microscopic examination of blood smears and PCR test as control, ICT was used to detect 89 patients suspected of having malaria. Results Compared with microscopic examination of blood smears, PCR test and ICT had positive coincidence rate of 93.4% and 86.4%, sensitivities of 90.16% and 83.33% and specificities of 92.86% and 91.30%, respectively. ICT Malaria P.f/P.v test was 100% sensitivity for infections with >100 parasites/μl, 72.73% with <100 parasites/μl, and 66.67% with <50 parasites/μl. Conclusion ICT could be used for diagnosis of malaria in China, but it should not replace conventional microscopic method.

关 键 词：诊断 疟疾 免疫层析试验 镜检法 聚合酶链反应 

分 类 号：R446.6[医药卫生—诊断学]