尿脱落细胞中细胞分裂周期蛋白6基因的检测  

Detection of cdc6 gene in exfoliate cell in urine

作　　者：安成[1] 白焱[1] 张秀敏[1] 杨振华[2] 张曼[1] 

机构地区：[1]铁道部北京铁路总医院临床检验中心 [2]铁道部北京铁路总医院卫生部临床检验中心,北京100038

出　　处：《中华检验医学杂志》2002年第1期35-37,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　通过对膀胱癌患者尿液脱落细胞中DNA复制起始复合物中的关键蛋白—细胞分裂周期蛋白 6(CDC6)的检测 ,探讨可早期发现 ,常规筛查膀胱癌 ,且不给患者带来痛苦的方法。方法　留取膀胱癌及其他泌尿系疾病患者的新鲜全程尿液 2 0 0ml,离心获得尿沉渣标本 ,提取总RNA ,经逆转录聚合酶链反应 (RT PCR) ,检测cdc6基因的表达。结果　 3 0例膀胱癌患者中 ,cdc6阳性 2 8例 ,阴性 2例 ,阳性符合率为 93 .3 %。 3 0例其他泌尿系疾病患者和健康人尿液中 ,cdc6检出率16 7% ;与尿液脱落细胞学检查相比较 ,cdc6RT PCR具有较高的敏感性 (P <0 .0 5 )。Objective DNA replication increases during tumor cells limitlessly proliferate. To develop a method that can early diagnose and routine screen out bladder cancer, but it does not impair patients by detecting cdc6 in exfoliate cell in urine of patients with bladder cancer. Methods 200 ml fresh urine of patients with bladder cancer or non bladder cancer who voided urine after morning was collected and centrifugated for urine sediment, then total RNA was extracted with TRIZOL reagent from it and detected the expression of cdc6 gene by RT PCR. Results 28 samples were positive and 2 samples were negative in 30samples of bladder cancer, positive rate was 93.3%. Positive rate was 16.7% in 30 samples of non bladder cancer. Compared with the sensitivity of urine cytology, that of cdc6 RT PCR increases markedly ( P <0.05). Conclusion Cdc6 gene expression obviously increases in the urine exfoliated cell of bladder cancer patients.

关 键 词：膀胱肿瘤 细胞分裂周期蛋白6 逆转录聚合酶链反应 基因表达 CDC6 尿液 脱落细胞 

分 类 号：R446.12[医药卫生—诊断学]