人脐静脉内皮细胞清除补体攻膜复合物的机制  

Removal pathway of complement membrane attack complex and its rate of disappearance in human umbilical vein endothelial cells

作　　者：周镜然[1] 朱锡华[1] 白云[1] 姜曼[1] 黎万玲[1] 

机构地区：[1]第三军医大学免疫学教研室,重庆400038

出　　处：《免疫学杂志》2002年第1期6-8,16,共4页Immunological Journal

基　　金：国家自然科学基金资助项目 (397890 10 );国家重点基础研究"973"项目资助课题 (G19990 5 4 2 0 0 )

摘　　要：目的　探明内皮细胞清除补体攻膜复合物 (MAC)的途径及其清除动力学。方法　原代培养的人脐静脉内皮细胞以RH4 14荧光标记质膜双层 ,0℃组装亚溶破剂量的MAC ,37℃复苏后 ,LSCM实时监测MAC沉积诱导的质膜囊泡化形成和胞吞、胞吐情况。流式细胞仪定量检测内皮细胞表面MAC抗原的清除情况。结果　MAC沉积后 ,内皮细胞有活跃的质膜囊泡化形成 ,囊泡以胞吞和胞吐 2种方式离开细胞 ,并以前者占优。 37℃条件下 ,内皮细胞清除表面MAC的半衰期约为 5min。结论　内皮细胞可通过胞吞和胞吐 2种机制清除细胞表面沉积的MAC ,并以胞吞方式为主。Objective To investigate the pathway of complement membrane attack complex removal and its rate of disappearance in human umbilical vein endothelial cells. Methods The elimination process of HUVECs on deposited MAC was observed by using laser scanning confocal microscope (LSCM), and the removal dynamics of MAC neoantigen removal was also detected with flow cytometry. Results A large amount of vesicles formed from plasma membrane can been seen upon deposition of sublytic MAC. The vesicles left cell surface in both endocytosis and exocytosis process, and the former mechanism was predominant. The half life of MAC neoantigen was about 5 min in HUVECs at 37 ℃ .Conclusions Human umbilical vein endothelial cells remove membrane attack complex by both endocytosis and exocytosis process. [

关 键 词：补体 膜攻击复合物 血管内皮细胞 囊泡化形成 脐静脉 

分 类 号：R392.11[医药卫生—免疫学]