人白细胞介素18的原核表达及多克隆抗体的制备  

Prokaryotic expression of human interleukin 18 and the preparation of polyclonal antibody

作　　者：裴冬生[1] 孙亚锋[1] 傅奕[1] 胡书群[1] 陆梁[1] 赵惠仁[1] 

机构地区：[1]徐州医学院生物化学与分子生物学研究中心,江苏徐州221002

出　　处：《免疫学杂志》2002年第1期48-50,共3页Immunological Journal

摘　　要：目的　在原核系统中表达并纯化人白细胞介素 18(hIL 18) ,制备兔抗人白细胞介素 18多克隆抗体。方法　用RT PCR扩增出hIL 18的cDNA ,克隆到原核表达载体pJW2中 ,转化大肠杆菌JM10 1中诱导表达并纯化。用纯化的人白细胞介素 18免疫新西兰兔制备多克隆抗体。结果　克隆得到序列正确的hIL 18cDNA ,与载体连接后转化入大肠杆菌JM10 1中诱导表达并成功纯化 ,并用纯化的人白细胞介素 18成功制备了兔抗人白细胞介素 18多克隆抗体。Objective To express and purify the recombinant human interleukin 18 (rhIL 18) in prokaryotic system and to prepare the rabbit anti human interleukin 18 polyclonal antibody.Methods The cDNA of hIL 18 was amplified by RT PCR and was cloned into the expression vector pJW2 and transformed into the E.coli JM101. The purified recombinant protein was used to immune the rabbit for preparing polyclonal antibody.Results The cDNA of hIL 18 was sequenced and transformed into the E.coli. The recombinant human IL 18 (rhIL 18) was expressed efficiently in inclusion bodies and was successfully purified, the polyclonal antibody was successfully prepared with the purified recombinant human IL 18.Conclusion The rabbit anti human interleukin 18 polyclonal antibody can be used in further studies. [

关 键 词：白细胞介素18 原核表达 多克隆抗体 

分 类 号：R392[医药卫生—免疫学]