链脲佐菌素诱导的糖尿病大鼠外周血白细胞iNOS-mRNA表达的变化  

Alteration of leukocyte iNOS-mRNA expression in rat with diabetic mellitus induced by streptozocin

作　　者：李瑞峰[1] 郭成浩[1] 陈融[1] 李莉[1] 孔乐凯[1] 王建丽[1] 胡维诚[1] 

机构地区：[1]山东大学医学院病理生理教研室,山东济南250012

出　　处：《中国病理生理杂志》2001年第12期1240-1242,I004,共4页Chinese Journal of Pathophysiology

基　　金：山东省卫生厅资助课题 ( 99CA1CAA11)

摘　　要：目的 :研究链脲佐菌素 (STZ)诱导的糖尿病大鼠胰岛 β细胞的功能与外周血白细胞iNOS -mRNA表达的关系。方法 :Wistar大鼠随机分为对照组 10只 ;实验组 15只。测定血糖、血浆胰岛素 ;用原位杂交方法检测外周血白细胞、肝、肺等组织中iNOS -mRNA表达 ,观察白细胞iNOS -mRNA阳性细胞率。结果 :用STZ腹腔注射损伤胰岛β细胞并导致胰岛功能衰竭 ,3d后血糖由 ( 8 95± 1 80 )mmol·L-1升高至 ( 2 2 84± 4 90 )mmol·L-1,血浆胰岛素由 ( 81 76± 2 0 12 )mU·L-1降至 ( 5 8 92± 18 2 0 )mU·/L-1。正常大鼠外周血白细胞未见iNOS -mRNA表达 ,而STZ诱导的糖尿病大鼠外周血白细胞iNOS -mRNA表达高度增强。结论 :STZ诱导的胰岛 β细胞损伤与白细胞的iNOS -mRNA表达存在正相关关系。本实验为检测外周血白细胞某些信号通路提供了简便易行的原位杂交试验方法。AIM: To clarify the relationship between expression of leukocyte iNOS-mRNA and pancreatic islet function in the diabetic rats induced by streptozocin(STZ). METHODS: Wistar rats were randomly divided into the control group ( n =10) and the diabetic group ( n =15). Expression of iNOS-mRNA in the peripheral blood leukocyte, liver and lung were detected with in situ hybridization and the blood sugar and insulin were also measured. RESULTS: It showed that the blood glucose content increased from (8 95±1 80) to (22 84±4 90) mmol·L -1 ,however, the plasma insulin content decreased from (81 76±20 12) to (58 92±18 20) mU·L -1 at the third day after the β cell was disfunctioned by STZ injection. No expression of leukocyte iNOS-mRNA in normal rat was detected. The percent rate of positive cells were significantly increased in the rats with diabetes. CONCLUSION: The expression of leukocyte iNOS-mRNA is positively related to the damage of β cells caused by STZ.

关 键 词：糖尿病 白细胞 一氧化氮合酶 链尿佐菌素 

分 类 号：R587.1[医药卫生—内分泌]