油酸钠与紫外线联用灭活血浆中病毒的研究  

STUDY ON INACTIVATION OF VIRUS IN PLASMA BY COMBINATION OF SODIUM OLEATE AND ULTRAVIOLET RAYS

作　　者：刘蓉[1] 沈德林[1] 张文福[1] 袁庆霞[1] 钟儒波[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国消毒学杂志》2001年第4期208-213,共6页Chinese Journal of Disinfection

基　　金：国家自然科学基金(39700127)资助

摘　　要：采用细胞感染法，对油酸钠与短波紫外线（ＵＶＣ）联用灭活血浆中病毒进行了研究。结果，３２００μＷ／ｃｍ２　ＵＶＣ与３．０ｍｇ／ｍｌ油酸钠联合作用３０ｍｉｎ或３２００μＷ／ｃｍ２　ＵＶＣ与２．０ｍｇ／ｍｌ油酸钠联合作用４０ｍｉｎ，可使血浆中水泡性口炎病毒（ＶＳＶ）滴度下降 ６．３３～６．４４ｌｇ ＴＣＩＤ５０，辛德比斯病毒滴度下降６．０９—６．３４ｌｇ ＴＣＩＤ５０。且两者联用协同系数（Ｔ／Ｅ）远大于１，有明显增效作用。但该方法使Ｍ１３噬菌体和ｆ噬菌体滴度下降≤３ｌｇ ＰＦＵ。用 ＲＴ－ＰＣＲ扩增 ＶＳＶ编码 Ｎ蛋白的一段核酸，研究此消毒方法对ＶＳＶ核酸的影响。结果，经ＵＶＣ照射的含ＶＳＶ血浆目的片段扩增阴性，油酸钠处理的含ＶＳＶ血浆扩增阳性。表明ＵＶＣ照射作用于ＶＳＶ核酸，使之断裂，从而灭活病毒，而油酸钠对ＶＳＶ的这段核酸无破坏作用。Inactivation of virus in plasma by combination of sodium oleate and short-wave ultraviolet rays（UVC) was studied using cell infection method. The results showed that expo- sure to combination of 3200 μW/ cm2 UVC and 3.0 mg/ ml sodium oleate for 30 min or 3200μW/ cm2 UVC and 2.0 mg / ml sodium oleate for 40 min reduced the titer of vesicular stomatitis virus（VSV) in plasma by 6.33-6.44 lg TCID50 and that of Sindbis virus（SV) by 6.09-6.34 lg TCID50. The synergetic coefficient（T/E) of these two inactivating agents was higher than I with obvious synergetic effect. However, this method reduced the titers of M13 bacteriophage and f2 bacteriophage by≤31g PFU. A segment of the nucleic acid encoding N protein of VSV was amplified with RT-PCR to study the effect of this disinfecting method on VSV nucleic acid. The result showed that amplification of the target segment in the plasma containing VSV exposed to UVC was negative, while amplification of the plasma containing VSV treated with sodium oleate was positive, indicating that UVC irradiation caused break- age of VSV nucleic acid and thus inactivated the virus, while sodium oleate had no destructive effect on this segment of nucleic acid of VSV.

关 键 词：血浆消毒 油酸钠 短波紫外线 水泡性口炎病毒 细胞感染法 

分 类 号：R187.2[医药卫生—流行病学] R187.4[医药卫生—公共卫生与预防医学]