中外十个猪种H-FABP基因遗传变异的研究  

Research on Genetic Variation of Heart Fatty Acid-Binding Protein Gene in Ten Pig Breeds

作　　者：林万华[1] 黄路生[1] 任军[1] 邓素华[1] 王文君[1] 刘宝生[1] 周利华[1] 陈从英[1] 

机构地区：[1]江西农业大学江西省动物生物技术重点开放实验室,南昌330045

出　　处：《Acta Genetica Sinica》2002年第1期12-15,共4页

基　　金：国家 973项目 (G2 0 0 0 0 16 10 0 );国家优秀青年教师基金资助

摘　　要：利用PCR RFLP技术 (限制性内切酶采用HinfⅠ ,HaeⅢ和MspⅠ )检测了杜洛克、长白、大约克、南昌白、二花脸、梅山、玉山黑猪、乐平花猪、金华两头乌及上高两头乌 10个猪种共计 5 6 1头猪在心肌脂肪酸结合蛋白 (H FABP)基因位点的遗传变异。结果表明 :(1)在HinfⅠ RFLP位点上 ,南昌白猪均为HH纯合子 ,而其他猪种在此位点上均存在变异 ,外来品种以纯合子HH居多 ;(2 )在HaeⅢ RFLP位点上 ,外来猪种杜、长、大及培育品种南昌白猪均在该位点表现为多态 ,但中国 6个地方猪种在该位点均无变异 ;(3)在MspⅠ RFLP位点上 ,仅有杜洛克在该位点表现为多态。而所有检测的地方猪种均表现为AADD__型。The genetic variation of heart fatty acid binding protein ( H FABP ) gene in 561 pigs including Duroc, Landrace, Large Yorkshire, Nanchang white pig, Erhualian, Meishan, Yushan black pig, Leping spotted pig, Jinhua black head hind pig and Shanggao black head hind pig were detected by PCR RFLP with Hin fⅠ , Hae Ⅲ and Msp Ⅰ. The results showed as follows:(1) Nanchang white pig presented only HH genotype while other breeds varied at the Hin fⅠ RFLP site; (2) The exotic breeds including Duroc, Landrace, Large Yorkshire and the native breed Nanchang white pig were proved to be polymorphic while the five Chinese local breeds presented no variation at the Hae Ⅲ RFLP site; (3) Among the tested breeds only Duroc presented variation at the Msp Ⅰ RFLP site. It is noted that all the Chinese local breeds present as AADD __genotypes.

关 键 词：猪 H-FABP基因 遗传变异 品种 

分 类 号：S828.2[农业科学—畜牧学]