外源RNA对小鼠白蛋白基因表达及DNaseⅠ敏感性的影响  

Influence of Exogenous RNA upon Expression of Mouse Albumin Gene and Its Sensitivity to DNaseⅠ

作　　者：张红艳[1] 刘爱京[1] 刘福英 王秀芳 吕占军 

机构地区：[1]河北医科大学实验动物学部,石家庄050017

出　　处：《Acta Genetica Sinica》2002年第1期26-29,T001,共5页

基　　金：国家 95攻关课题‘96 -A2 3-0 6 -0 3’~~

摘　　要：兔肝RNA诱导培养的小鼠成纤维细胞 ,大鼠肝RNA注射入小鼠前列腺 ,用免疫组织化学染色检测外源RNA对小鼠白蛋白基因表达的影响 ;不同RNA诱导培养的小鼠成纤维细胞 ,提取细胞核 ,DNaseⅠ消化 ,PCR法扩增小鼠白蛋白基因 ,检测白蛋白基因消化情况。Cultured mouse fibroblasts were treated with rabbit liver RNA. Rat liver RNA was injected into mouse prostates. Influence of exogenous RNA upon expression of mouse albumin gene was detected by immunohistochemical staining. Nucleuses of cultured mouse fibroblasts treated with different RNAs were isolated and digested with DNase Ⅰ. Mouse albumin gene was amplified by PCR to detect levels of its digestion. It was found that exogenous RNAs could increase the sensitivity of mouse albumin gene to DNase Ⅰ digestion and promote its expression.

关 键 词：外源RNA DNASEI 敏感性 小鼠 Alb基因 白蛋白 基因表达 

分 类 号：Q786[生物学—分子生物学]