鸡类胰岛素生长因子-Ⅱ基因单核苷酸多态与生长、屠体性状相关性的研究  

Single Nucleotide Polymorphism Analysis in Chicken Insulin like Growth Factor- Ⅱ Gene and Its Associations with Growth and Carcass Traits

作　　者：颜炳学[1] 李宁[1] 邓学梅[2] 胡晓湘[1] 刘兆良[1] 赵兴波[2] 连正兴[2] 吴常信[2] 

机构地区：[1]中国农业大学农业生物技术国家实验室,北京100094 [2]中国农业大学动物科学技术学院,北京100094

出　　处：《Acta Genetica Sinica》2002年第1期30-33,共4页

基　　金：国家重点基础研究发展规划项目资助 (编号 :G2 0 0 0 0 16 1)~~

摘　　要：以鸡类胰岛素生长因子 -Ⅱ (IGF -Ⅱ )基因作为控制鸡生长、屠体性状主基因的候选基因 ,以明星肉鸡和丝毛乌骨鸡杂交产生的F2 代鸡群为实验材料。通过PCR -RFLP方法对IGF -Ⅱ基因进行多态性检测。在该基因外显子 2中发现一处碱基突变 ,可由内切酶Aci-Ⅰ酶切证实 ,但未导致氨基酸改变。对F2 鸡群个体进行了基因型鉴定 ,结果经方差分析显示 :该基因对一些生长、屠体性状有显著性影响 (如胸角宽、腺胃重、半净膛重等 )。表明该基因可能调控鸡生长、屠体性状的表达 ,或与控制生长、屠体性状的主基因连锁。In this experiment, F 2 chicken derived from Broilers crossing with Silky was used to study the effect of insulin like growth factor Ⅱ gene on growth and carcass traits. The partial gene was amplified by two pairs of primers, and single nucleotide polymorphism (SNPs) was detected by the technique of restriction fragment length polymorphism (RFLP), and then confirmed by DNA sequencing. The mutation was found in the exon 2 of the gene, and can be clarified by cutting of restriction enzyme Aci- Ⅰ. The result of least square analysis showed the gene was significantly related with growth and carcass traits. It implied that the insulin like growth factor Ⅱ gene could be a genetic locus or linked to a major gene affecting greatly the growth and carcass traits in chicken.

关 键 词：鸡 类胰岛素生长因子-Ⅱ基因 单核苷酸多态 屠体性状 生长性状 

分 类 号：S831.1[农业科学—畜牧学]