两个人工雌核发育红白锦鲤群体的RAPD标记分析  

RAPD MARKER ANALYSIS OF TWO ARTIFICIAL GYNOGENETIC POPULATIONS IN RED-WHITE ORNAMENTAL CARP

作　　者：贾海波[1] 周莉[2] 桂建芳[2] 

机构地区：[1]湖北大学生命科学院,武汉430062 [2]中国科学院水生生物研究所

出　　处：《水生生物学报》2002年第1期1-7,共7页Acta Hydrobiologica Sinica

基　　金：国家重点基础研究项目 ( G1 9990 5 390 8) ;武汉市科技计划项目 ;中国科学院基础研究项目 ( KJ95 1 -B1 -6 0 7-3)资助

摘　　要：采用 RAPD方法 ,对两个人工雌核发育红白锦鲤群体进行了多态性及分子标记分析。结果表明 ,同一雌核发育群体具有基本一致的扩增产物 ,而不同雌核发育群体间的扩增产物则有较大不同 ;并从 3 0个随机引物的扩增谱带中找到了 7个引物 (Opo- 7,Opo- 9,Opo- 1 2 ,Opo- 1 4,Opj- 4,Opj- 8和 Opj- 1 0 )的扩增谱带可以作为两个不同雌核发育群体间的遗传标记。由 UPGMA聚类法构建的分支系统树清晰地反映了两个雌核发育群体及其个体间的相互关系。Genetic heterogeneity and molecular markers were analyzed in two artificial gynogenetic populations of Red-white ornamental carp (Cyprinus carpio L.) by RAPD technique, using 30 10-nucleotide primers. Similar banding patterns were observed among individuals within each gynogenetic population, whereas obvious differences were detected between the two gynogenetic populations. 7 primers, such as Opo-7, Opo-9, Opo-12, Opo-14, Opj-4, Opj-8 and Opj-10 were observed to produce specific bands, and these bands could be used as molecular markers for discriminating the two different gynogenetic populations. Average genetic distances within and among the two populations clearly indicated their origin and relationship. A phylogenetic tree was constructed using UPGMA cluster analysis based on a total of 1710 distinguishable fragments (171 per individual). All individuals in the first gynogenetic population were clustered into one group, while all the individuals originated from the second were joined into another group.

关 键 词：锦鲤 人工雌核发育 RAPD 遗传标记 

分 类 号：S965.8[农业科学—水产养殖]