嗜水气单胞菌和河弧菌二联疫苗对鲫的免疫效果  

DEVELOPMENT AND IMMUNE EFFICACY OF A BIVALENT ANTI-AEROMONAS HYDROPHILA AND ANTI-VIBRIO FLUVIALIS VACCINE IN CARASSIUS AURATUS LINNAEUS

作　　者：李爱华[1,2] 吴玉深[1,2] 蔡桃珍[1,2] 卢全章[1,2] 

机构地区：[1]中国科学院水生生物研究所 [2]淡水生态与生物技术国家重点实验室,武汉430072

出　　处：《水生生物学报》2002年第1期52-56,共5页Acta Hydrobiologica Sinica

基　　金：中国科学院知识创新工程项目 KSCX2 -1 -0 4 ;生物学及生物技术特别支持费资助

摘　　要：在采用正交试验设计优化了 Aeromonas hydrophila和 Vibrio fluvialis二联全菌疫苗制备工艺的基础上 ,研究了采用该工艺所制备的疫苗对鲫的免疫保护效果。结果表明注射免疫鲫4周后 ,抗 A.hydrophila和 V.fluvialis血清凝集效价分别为 :1∶ 4— 1∶ 3 2和 1∶ 2— 1∶ 1 6 ,平均都为 1∶ 1 0 ,但对攻毒均具有有效的保护作用。浸泡免疫、高渗浸泡免疫或加多糖浸泡免疫在免疫 4周后 ,对 A.hydrophila攻毒均无保护作用 ;然而 ,加多糖浸泡免疫对 V.fluvialis攻毒可产生有效的保护。免疫扩散试验表明 ,在煮沸并经超声波处理后的 A.hydrophila或V.fluvialis菌液中均存在两种优势抗原。Aeromonas hydrophila and Vibrio fluvialis are the causative agents pathogenic bacterial of haemorrhagic septicemia of a variety of farmed fish in China. A bivalent vaccine against these two pathogens was being developed by an orthogonal array design. The optimized bivalent vaccine could elicit effective protection from experimental challenge of A. hydrophila for C. auratus 4 weeks after immunized by i.p injection, but not by neither immersion vaccination nor hyperosmotic infiltration with or without supplement of polysaccharide. The vaccine was also protective against the challenge of V. fluvialis when fish was immunized by i.p injection and immersion vaccination plus polysaccharide. At challenge time, serum agglutination titers from fish injected with the bivalent vaccine were 1∶4-1∶32, 1∶2-1∶16, results of immunodiffusion indicated that there were two dominant antigens in boiled and sonicated V. fluvialis and A. hydrophila vaccine.

关 键 词：二联疫苗 嗜水气单胞菌 河弧菌 鲫鱼 免疫效果 

分 类 号：S941.42[农业科学—水产养殖] S942.5[农业科学—水产科学]