增生瘢痕中转化生长因子基因的表达  

Change of gene expression of transforming growth factor-β1, Smad 2 and Smad 3 in hypertrophic scars skins

作　　者：陈伟[1] 付小兵[1] 孙同柱[1] 孙晓庆[1] 赵志力[1] 盛志勇[1] 

机构地区：[1]解放军第三○四医院全军烧伤研究所,北京100037

出　　处：《中华外科杂志》2002年第1期17-19,共3页Chinese Journal of Surgery

基　　金：国家重大基础研究规划资助项目 (G19990 5 42 0 4) ;国家杰出青年科学基金 ( 395 2 5 0 2 4);全军九五医学科研基金重点课题( 98Z0 90 )资助项目

摘　　要：目的　探讨转化生长因子 (TGF) β1和转录因子Smad 2 ,Smad 3三种基因影响增生性瘢痕形成和皮肤伤口无瘢痕愈合的调节机制。方法　 3 2份被检测标本中包括增生性瘢痕 8份 ,其自体正常皮肤组织 8份 ,胎儿和成人皮肤组织各 8份。用逆转录 多聚酶链反应方法 (RT PCR)检测上述基因在不同的组织细胞内的表达变化规律。结果　TGF β1、Smad 2和Smad 3基因在增生性瘢痕、胎儿和成人皮肤组织细胞内都有表达。在所检测 8对增生性瘢痕和其对应正常皮肤细胞中 ,这 3种不同基因在增生性瘢痕细胞中的表达量高于正常皮肤细胞的组数分别为 :TGF β1基因有 5对 ,Smad 2基因有8对 ,Smad 3基因有 5对。在胎儿皮肤细胞内 ,TGF β1的mRNA含量明显低于成人皮肤细胞 (t =2 2 0 4 ,P <0 0 5 ) ,差异有显著意义 ;Smad 3基因表达也呈相似的变化规律 ,mRNA含量显著低于成人皮肤细胞 (t=4 2 69,P <0 0 1) ,差异有非常显著意义 ;而Smad 2基因的mRNA含量却明显高于成人皮肤细胞 (t=6 685 ,P <0 0 1) ,差异亦有非常显著意义。结论　TGF β1和它的下游信号分子Smad2 ,Smad 3可能与增生性瘢痕形成密切相关。在增生性瘢痕细胞内 ,这 3种基因高表达可诱导胞外基质大量沉积 ,加速成纤维细胞增殖 ,促进组织纤维化。TGF β1和Smad 3基?Objective To explore the regulatory mechanisms of transforming growth factor β1(TGF β1) and two transcriptional factors Smad 2,3 on hypertrophic scar formation and fetal scarless healing. Methods Thirty two cases were detected to compare the gene expression of TGF β1, Smad 2 and Smad 3 with RT PCR. Among these cases, there were 8 cases of hypertrophic scars, 8 cases of control skins, 8 cases of fetal skins, and 8 cases of adult skins. Results TGF β1, Smad 2 and Smad 3 gene expression could all be detected in hypertrophic scars, and in fetal and adult skins. Among 8 groups examinated in this experiment(each group comprised a hypertrophic scar and its corresponding normal skin), there were 5, 8 and 5 groups in which TGF β1, Smad 2 and Smad 3 gene expressions were higher in hypertrophic scars than in normal skins respectively. The fetal skins showed significantly lower level of TGF β1 and Smad 3 gene expression compared with adult skins( t =2 204, P <0 05 and t =4 269, P <0 01 respectively), while mRNA contents of Smad 2 were obviously higher in fetal skins than in adult skins( t =6 685, P <0 01). Conclusions TGF β1 and its downstream signal molecules Smad 2, Smad 3 might be involved in hypertrophic scar formation. Higher gene expression of TGF β1, Smad 2 and Smad 3 in hypertrophic scars might lead to stimulating extracellular matrix deposition, inducing fibroblast proliferation and accelerating fibrogenesis. Lower mRNA contents of TGF β1 and Smad 3 in fetal skins compared with adult skins might be associated with fetal scarless healing.

关 键 词：转化生长因子Β 增生瘢痕 转录因子 伤口愈合 TGF-Β1 SMAD2 SMAD3 

分 类 号：R622[医药卫生—整形外科]