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机构地区:[1]哈尔滨医科大学第二临床医学院整形外科,150086
出 处:《中华外科杂志》2002年第1期27-29,T002,共4页Chinese Journal of Surgery
基 金:国家自然科学基金资助项目 ( 30 170 970 )
摘 要:目的 为将内皮细胞和平滑肌细胞种植于脱细胞血管基质 (ACTM)上形成组织工程化血管提供实验依据。方法 用曲拉通 (Triton)X 10 0等试剂制备猪胸主动脉ACTM ,并寻找曲拉通X 10 0的最佳浓度。取家猪的新鲜去除外膜胸主动脉 5 6根 ,随机分成 7组 ,每组分别浸入不同浓度的曲拉通X 10 0中作用 14 4~ 2 4 0h。标本作HE弹力纤维染色 ,大体、光镜及透射电镜观察。结果 经上述试剂处理后 ,光镜及透射电镜检查显示 ,制备出的猪胸主动脉脱细胞血管基质由胶原纤维、弹力纤维和某些不可溶的、变性细胞器构成。曲拉通X 10 0最佳浓度为 1%。时间为 176 2 5h± 5 5h。结论 本方法可成功制备猪胸主动脉ACTM ,1%曲拉通X 10Objectives To investigate the method of preparing porcine thoracic aortas acellular tissue matrix(ACTM) by trypsin,EDTA and Triton X 100 and to find the best concentration of X 100. Methods A total of 56 roots of fresh thoracic aortas (without adventitial tissue) from 80 kg 100 kg tame pigs were divided randomly into > groups, each containing 8 roots. Every vessel was put into a 50 ml centrifugal tube with a solution of 0 1%trypsin+0 02EDTA in PBS for 24 h. After that, each group was separately immerged into a solution of 0 1%, 0 2%, 0 5%, 1 0%, 2 0%, 5 0%, 10 0% Triton X 100 for 144 h 240 h. Specimens were taken every 6 h. Specimens were stained with haematoxylin eosin and observed grossly under the light and transmission electron microscopy. Results Light and transmission electron microscopy revealed that ACTM was composed of insoluble collagen, elastin, and some insoluble metamorphic organelles. The best concentration of Triton X 100 was 1% at the time of 176 25 h±5 5 h.Conclusions Porcine thoracic aortas ACTM can be obtained successfully through this procedure. Triton X 100 is a good reagent for preparing vessel ACTM.
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