重组人锰超氧化物歧化酶工程菌高效表达培养工艺的研究  

Studies on cultural technology of high expression of the recombinant human maganese-superoxside dismutase in E.coli

作　　者：张艳红[1] 廖晓全[1] 范立强[1] 袁勤生[1] 

机构地区：[1]华东理工大学生物工程学院国家重点实验室,上海200237

出　　处：《中国药学杂志》2002年第1期59-62,共4页Chinese Pharmaceutical Journal

摘　　要：目的　确定重组人锰超氧化物歧化酶 (rhMn SOD)工程菌高效表达的最适培养工艺条件。方法　通过摇瓶培养研究了包括接种量、诱导时机、Mn2 + 浓度、诱导后培养时间、发酵过程中 pH变化以及初始 pH值对发酵的影响。 结果　该工程菌的最佳接种量为 3%,最佳诱导时机为接种后 3h ,确定Mn2 + 浓度为 6 0 0 0 μmol·L-1,诱导后培养 5h ,在培养过程中当pH为6 .83时外源蛋白可获得高效表达 ,初始 pH确定为 8.0。 结论 :诱导和培养基的 pH是影响工程菌发酵的主要因素 ,尤其是Mn2 + 的加入表达有活性的rhMn SOD是必需的。OBJECTIVE: To define the suitable culture technology of recombinant human maganese-superoxide dismutase. METHODS: The conditions including inoculum quantities, induced time, concentration of Mn2+, culture time after induced, changes of pH during the culture and original pH of culture by culture in shaking-bottle were investigated. RESULTS: The suitable culture technology conditions were 3 % inoculum quantities, induced by 6 000 μmol&middotL-1 Mn2+ for 3 hours after inoculum, and cultured 5 hours after induced. The exogenous protein expressed highly at pH 6.83 and the original pH was 8.0. CONCLUSION: Induction and pH is the key factor that affect the fermentation of the engineer bacterium, especially the additition of the Mn2+ is necessary to the expression of the active rhMn-SOD.

关 键 词：重组人锰超氧化物歧化酶 工程菌 培养工艺 rhMn-SOD 

分 类 号：Q814[生物学—生物工程]