人骨骼肌细胞体外分化过程及特征  

STUDY ON DIFFERENTIATION AND CHARACTERIZATION OF CULTURED HUMAN SKELETAL MUSCLE CELLS

作　　者：马焰[1] 沈定国[2] 孙月芬[1] 赵民[1] 高伟[1] 陈刚[1] 

机构地区：[1]北京军医学院 [2]解放军总医院

出　　处：《解放军医学杂志》2002年第2期137-138,T001,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"九五"计划面上项目基金资助课题 (编号 98M1 1 8)

摘　　要：为研究人骨骼肌细胞(HSMC)体外分化模式及其生物学、形态学、生化学特征 ,在体外培养HSMC ,并进行免疫组化鉴定、细胞生物学和形态学观察、肌酸激酶 (CK)及蛋白质含量测定。结果发现 ,体外培养的HSMC能达到高度分化 ;CK活力、肌细胞蛋白质合成与其分化、成熟程度及肌管的功能状态呈正相关。提示HSMC体外重新分化过程与在体相似。The aim was to establish the differentiating model ' in vitro ' and observe the characteristics of human skeletal muscle cells (HSMC). This study developed an ' in vitro ' technique of preparing cultures of HSMC obtained from 8 normal subjects. Culture of HSMC was identified with immunohistochemistry, and the characteristics in cytobiology , cytobiochemistry and cytomorphology were observed. CK activity and protein synthesis were measured.The results showed that cultured HSMC could highly differentiate, and there was positive correlations between protein synthesis, CK specific activity and the differentiating potency of HSMC. It suggested that the in vitro differentiation process of HSMC was similar to its in vivo differentiation process.

关 键 词：骨骼肌 细胞培养 细胞分化 肌酸激酶 体外分化 HSMC 

分 类 号：Q21[生物学—细胞生物学]