衰老和有氧运动对大鼠肝脏线粒体DNA突变的影响  

The Effect of Aging and Aerobic Exercise on Mitochondrial DNA Deletions in Rat Liver

作　　者：张勇[1] 时庆德[2] 聂金雷[1] 沙继斌[1] 杨锡让 刘树森[3] 

机构地区：[1]天津体育学院运动医学研究所,天津300381 [2]北京体育大学人体运动科学系 [3]中国科学院动物研究所生物膜与膜生物工程国家重点实验室

出　　处：《中国运动医学杂志》2001年第4期352-354,372,共4页Chinese Journal of Sports Medicine

基　　金：天津市教委自然科学研究项目(NO.991601)

摘　　要：当前研究表明机体衰老与线粒体功能下降关系密切。其中线粒体ＤＮＡ突变可能是线粒体功能下降与活性氧产生增多的关键因素之一。本研究检测到２２月龄大鼠肝脏线粒体ＤＮＡ出现４８３４ｂｐ缺失，３个月的游泳训练明显降低同龄大鼠线粒体ＤＮＡ４８３４ｂｐ缺失发生率。研究同时发现老年大鼠肝脏线粒体复合体Ｉ和ＩＶ活性明显低于年轻大鼠，而有氧运动可显著升高老年运动大鼠线粒体酶活性。结果表明有氧运动能够减少线粒体ＤＮＡ突变发生，进而改善线粒体功能，这可能是有氧运动提高线粒体功能，延缓衰老的重要机制。Many studies indicate that aging is associated with the alteration of mitochondrial function. Mitochondrial DNA mutation probably plays the important role in the decrease of mitochondrial function and the increase of reactive oxygen species. In present experiment, we detected the 4834bp deletions of mitochondrial DNA in aged rat (22 months old) liver using PCR technique.Three-month swimming training reduced the frequency of mitochondrial DNA deletions in 22 months old rats. The activities of mitochondrial respiratory chain complex I and IV in aged rats' liver decreased significantly compared with that in young rats. Both of two complexes activities increased significantly in exercise training aged rats compared with aged rats. The results indicate that aerobic exercise can reduce the frequency of mitochondrial DNA deletions and preserve mitochondrial functions of rats at old age.

关 键 词：衰老 有氧运动 线粒体 DNA 动物实验 运动生物化学 

分 类 号：R87[医药卫生—运动医学]