焦磷酸测序鉴别猪线粒体细胞色素 b基因单倍型(英文)  

Haplotypes Detection of the Pig Mitochondrial Cytochrome B Gene by Pyrosequencing

作　　者：蒋思文[1] Elisabette Giuffra Keif Andersson 

机构地区：[1]华中农业大学,湖北武汉430070 [2]Department of Animal Breeding and Genetics Swedish University of Agricultural Science,Uppsala Biomedical Centre

出　　处：《中国兽医学报》2002年第1期44-46,共3页Chinese Journal of Veterinary Science

基　　金：国家"973"重大基础研究项目 (G2 0 0 0 -0 1610 5 )~~

摘　　要：为了鉴别猪线粒体细胞色素 b(cytochrom e B,cyt b)基因单倍型 ,选择 9个不同品种共 4 15头猪作为试验材料 ,采用实时 DNA测序新方法焦磷酸测序 ,分析了 cyt b单核苷酸多态性 (single nucleotide polymorphism s,SNPs)。分析结果显示 3种不同单倍型 E、A1和 A2。除 1头临高猪属于单倍型 A2外 ,其他 6个中国地方品种猪均属于单倍型A1;瑞典长白猪和大白猪存在单倍型 E和 A1,而皮特兰猪存在单倍型 E和 A2。此外 ,基于焦磷酸测序 ,在通城猪中发现了 1个新的 SNP位点。To detect porcine mitochondrial cytochrome b (cyt b) gene haplotypes, pyrosequencing, a real time DNA sequence method, was used to analyze SNPs selected a total of 415 pigs from 9 different breeds. Pyrosequencing analysis of cyt b gene displayed 3 distinct haplotypes E, A1, A2, respectively. Except for only 1 Qingping pig, the other Chinese pigs from 6 unrelated breeds only present the haplotype A1. Swedish Large White and Landrace pigs present the haplotype E and A1 whereas Pietrain pigs present the haplotype E and A2. In addition, we found a new SNP in Tongcheng pigs based on pyrosequencing.

关 键 词：线粒体DNA 细胞色素B基因 单倍型 焦磷酸测序 猪 

分 类 号：S828.2[农业科学—畜牧学]