高胆固醇饲养兔鹌鹑和大鼠诱发高血脂和动脉粥样硬化的特征  

Characteristics of Hypercholesterolemia and Atherosclerosis in Rabbits Quails and Rats Fed with High Cholesterol Diet

作　　者：陈凯[1] 张雁芳[1] 王倩[1] 山丽梅[1] 姜雨鸽[1] 慕邵峰[1] 刘淑红[1] 汪海[1] 

机构地区：[1]中国人民解放军军事医学科学院毒物药物研究所,北京100850

出　　处：《解放军药学学报》2001年第3期117-120,124,F003,共6页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家重点基础研究发展规划项目!No.G19980 5 1112。

摘　　要：目的　比较高胆固醇饲养兔、鹌鹑和大鼠诱发高血脂和动脉粥样硬化的特点。方法　长时期以不同含量的高胆固醇饲料喂养兔、鹌鹑和大鼠 ;采用生化检验技术测定血浆总胆固醇和甘油三脂等血脂变化 ;采用病理组织学方法观察主动脉粥样斑块形成的特点 ;采用扫描和透射电镜技术观察血管内皮细胞的形态学变化。结果与结论　兔每天饲喂胆固醇 0 .4g,1个月后 ,血浆总胆固醇比基础饲料喂养时升高 11.8倍 ;喂养 2～ 3个月后 ,血浆总胆固醇仍稳定在此水平上 ;喂养 3个月后 ,形成典型的动脉粥样硬化病理学改变 ;此规律在 3批慢性实验中得到证实。若每天饲喂胆固醇量增至 1.2g,血浆总胆固醇升高5 3.2倍 ;喂养 1个月可形成主动脉粥样硬化的斑块。在相同条件下 ,鹌鹑每天饲喂胆固醇 0 .0 5g ,血浆总胆固醇升高 7.2倍 ,3个月后形成典型的动脉粥样硬化病理学改变。大鼠每天每公斤体重饲喂胆固醇 0 .4g ,血脂升高 1.3倍 ,1个月后 ,主动脉内皮细胞有单核细胞粘附 ,IL - 8等细胞因子表达增高 。Aim To investigate the characteristics of hypercholesterolemia and atherosclerosis in rabbits,quails and rats fed with high cholesterol diet.Methods The rabbits, quails and rats were given diet with high cholesterol for 1～3 months. The levels of plasma total cholesterol (TC), triglyceride(TG),high density lipoprotein cholesterol (HDL C) and low density lipoprotein-cholesterol (LDL C) were determined with the biochemical techniques and the atherosclerosis was observed with pathological techniques. Morphological changes of vascular endothelial cells were observed under electronic microscopes.Results and Conclusion The rabbits were given cholesterol at 0.4g·d -1 for 1month, the values of TC were increased 11.8 times,the high levels kept stable for 2～3 months,and the typical pathological manifestation of atherosclerosis was observed after 3 months.This phenomenon was found in the three repeated experiments.If the rabbits were given cholesterol 1.2g·d -1 for 1month,the values of TC were increased 53.2 times, and the pathological manifestation of atherosclerosis was observed.Under the same experimental conditions,the quails were given cholesterol at 0.05g·d -1 for 2 months or more.The values of TC were increased 7.2 times,and the pathological manifestation of atherosclerosis was observed after 3 months.The rats were given cholesterol at 0.4g·kg -1 ·d -1 for 1 month,the values of TC were increased 1.3 times, and the initial manifestation of atherosclerosis was observed, including adhesion of monocytes into the cellular membrane of vascular endothelium, and the increased expression of the interleukin 8.

关 键 词：高脂血症 动脉粥样硬化 AS 高胆固醇 血管内皮细胞 动物实验 

分 类 号：R589.2[医药卫生—内分泌] R543.5[医药卫生—内科学]