次声作用下大鼠脑皮质内谷氨酸及其代谢型受体1亚型mRNA的变化  

Changes of Glu and mGluR1 mRNA in cerebral cortex of rats after infrasound exposure

作　　者：刘恩渝[1] 费舟[1] 章翔[1] 陈景藻[2] 李志刚[1] 刘先珍[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710033 [2]第四军医大学西京医院物理治疗与康复医学科,陕西西安710033

出　　处：《第四军医大学学报》2001年第23期2137-2140,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 ( 39870 6 73);高等学校骨干教师资助计划项目

摘　　要：目的　探讨 8Hz130 d B次声作用对大鼠脑皮质内谷氨酸 (glutamic acid,Glu)水平和代谢性谷氨酸受体 1亚型(m Glu R1) m RNA表达的影响 .方法　 SD大鼠 6 0只随机分为对照组及次声作用 1,7和 14次组 .次声作用组动物采用自制的次声压力仓用 8Hz,130 d B的次声按规定次数 ,每次作用 2 h.通过氨基酸分析仪测定各组脑皮质 Glu的含量 .利用地高辛标记寡聚核苷酸探针原位杂交技术与计算机图像分析系统 ,在光镜下分别观察 m Glu R1在大鼠脑皮质阳性神经元的表达以及相应皮质的病理变化 .结果　次声作用 1次组 ,脑皮质内 Glu的含量开始升高 ,7次组显著高于对照组 (P<0 .0 1) ,14次组低于正常值 ;次声作用 1次组 ,m Glu R1m RNA阳性神经元数量增加 ;7次组至峰值 (P<0 .0 1) ,14次组数目基本恢复到正常水平 .结论　形态学研究表明次声作用 7次组皮质浅层有部分神经元损伤 .次声脑损伤过程中 ,脑皮质内 Glu含量的升高曲线与 m Glu R1表达合成增加趋势基本吻合 ,提示 Glu可能通过其受体 m GluAIM To study the alterations of Glu and mGluR1 in brain cortex of rats after infrasound exposure. METHODS 60 SD rats were randomly divided into four groups: control group and 1 time, 7 times, 14 times infrasonic damage groups. Rats were exposed to 8 Hz 130 dB infrasound as designed, once for 2 h. The level of Glu in cortex was observed by microdetermination. The alterations of mGluR1 mRNA expression and pathology of cortex were observed under light microscope (×400) with digoxin labeled oligonucleotide probe in situ hybridization method and computer aided image analysis. RESULTS The content of Glu increased after infrasonic damage in 1 time group and was significantly more in 7 times group than that in control. The level of Glu was lower in 14 times group than that in control. The expression of mGluR1 mRNA positive neurons increased in cortex after infrasonic damage in 1 time group and was most in 7 times group. While the number of mGluR1 mRNA positive neurons in 14 times group nearly reached normal level. We observed that a part of neurons in cortex were injured in 7 times group. CONCLUSION Under infrasonic brain injury, the changes of Glu is coincided with the alteration in expression and production of mGluR1 in brain cortex. Our study indicate that neurotoxin of Glu with mGluR1 might play an important role in mechanism of infrasonic brain injury.

关 键 词：次声 脑损伤 代谢性谷氨酸受体 MRNA 

分 类 号：R651.15[医药卫生—外科学]