二次脑损伤后大鼠脑皮层代谢型谷氨酸受体亚型4mRNA的变化  

Changes of metabotropic glutamate receptor subtype 4 mRNA after diffuse brain injuries coupled with secondary brain insults in rats

作　　者：白红民[1] 费舟[1] 章翔[1] 李志刚[1] 刘恩渝[1] 刘先珍[1] 梁景文[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710033

出　　处：《第四军医大学学报》2001年第23期2141-2144,共4页Journal of the Fourth Military Medical University

基　　金：全军"九五"医学科研规划基金资助项目 ( 98M10 1);高等学校骨干教师资助计划项目

摘　　要：目的　研究弥漫性脑损伤 (DBI)及其合并二次脑损伤 (SBI)后大鼠脑皮层代谢型谷氨酸受体亚型 4(m Glu R4)的变化及意义 .方法　 SD大鼠随机分为正常对照组、假手术组、单纯 SBI组、单纯 DBI组及合并 SBI组 .在 Marmarou弥漫性脑损伤模型的基础上 ,制成 SBI模型 ,于伤后 1,6 ,12 ,2 4和 72 h进行 HE染色和代谢型谷氨酸受体 4亚型(m Glu R4) m RNA原位杂交 .结果　与正常对照组相比 ,假手术组和单纯 SBI组阳性神经元数以及信号灰度均无明显改变 (P>0 .0 5 ) ;与假手术组相比 ,单纯 DBI组和合并 SBI组 ,m Glu R4m RNA表达于脑损伤后 1h即有明显增加 (P<0 .0 1) ,单纯 DBI组在 6 h达到高峰 ,合并 SBI组于 12 h达到高峰 ,此刻与单纯 DBI组相比 ,合并 SBI组亦明显增加 (P<0 .0 1) .结论　 m Glu R4参与了 DBI和 SBI的病理生理过程 。AIM To study the changes and effects of metabotropic glutamate receptor subtype 4 (mGluR4) after diffuse brain injuries (DBI) coupled with secondary brain insults (SBI) in rats. METHODS 105 male SD rats were randomized into five groups: normal control group, sham operated group, SBI alone group, DBI alone group, DBI coupled with SBI group. The SBI model was made on a basis of Marmarou model. The brain sections were stained with H&E and the mRNA of mGluR4 were detected in 1, 6, 12, 24, 72 h after injuries by hybridization in situ . RESULTS Statistical analysis showed no significance between normal control group, sham operated group and SBI alone group ( P > 0.05 ). The animals exposed to DBI with/without SBI showed a significant expression of mRNA of mGluR4 compared to the sham operated animals 1 h after injuries ( P < 0.01 ). The expression reached the highest level in DBI alone group at 6 h. But it would still increase in DBI with SBI group 6 h after injuries, and reached the peak at 12 h. At this moment, the results showed a significant increase in DBI with SBI group rather than in DBI alone group ( P <0.01). CONCLUSION mGluR4 might play a role in the pathophysiology of DBI and SBI, which would provide a new method for severe brain injury therapy.

关 键 词：脑损伤 代谢型谷氨酸受体亚型4 病理生理 MRNA 

分 类 号：R651.15[医药卫生—外科学]