模拟高原梭曼中毒脑组织磷脂酶A_2等活性和含量的变化  

Changes of PLA_2,ATP,ADP,AMP and cAMP on Brain Tissues after Combined Soman and Hypoxia Injury

作　　者：王仕丽[1] 赵吉清[1] 吴强[1] 李云鹏[1] 董兆君[1] 魏相德[1] 

机构地区：[1]第三军医大学毒理学教研室,重庆400038

出　　处：《中国公共卫生》2002年第1期87-89,共3页Chinese Journal of Public Health

摘　　要：目的　探讨高原缺氧复合梭曼中毒脑组织中磷脂酶A2 (PLA2 )、腺苷酸、环磷酸腺苷的变化及其在脑损伤中的作用机制。方法　以Wistar大鼠为模型观察高原缺氧复合梭曼中毒后 12 ,2 4 ,4 8h大鼠脑水肿的发生率以及脑组织中PLA2 的活性、腺苷酸、环磷酸腺苷 (cAMP)的变化特点。结果　高原缺氧复合梭曼中毒后 2 4h脑组织伊文思蓝含量(EB)、PLA2 的活性、腺苷酸含量明显升高以及海马、小脑、皮层cAMP的含量明显升高。结论　高原缺氧复合梭曼中毒时 ,脑组织损伤具有早而重的特点。其具体机制可能与能量代谢抑制及衰竭和磷脂酶A2 和腺苷酸环化酶的激活有关。Objective To study the mechanism of the effect of PLA 2 on the damage of brain tissues after combined soman and hypoxia injury.Methods The changes in cAMP,ATP,ADP,AMP,PLA 2,Evans blue,water contents of brain tissue were studied in the Wister after the combined soman and acute hypoxia injury with microtitration,albumin labelled with Evans blue,RIA,and electron micropy for 12,24,48 hours.Results The changes of the content of cAMP,ATP,ADP,AMP,PLA 2,Evans blue were significantly higher at soman intoxicated group at hypoxia than at soman intoxicated group and control group at hypoxia.Conclusion The damage became more severe at combined soman and hypoxia injury,compared with soman or hypoxia injury,also the damage appeared earlier and more severe.It shows alteration of brain energy metabolism,activation of PLA 2,activation of cAMP and their correlation with neuronal damage.

关 键 词：梭曼中毒 脑组织 磷脂酶A2 活性 含量 缺氧 

分 类 号：R827.12[医药卫生—临床医学]