转染II类分子反式激活因子基因突变体抑制猪SLA的表达  

Depression of SLA Expression by Transfection of the Mutants of Class II Transactivator Gene

作　　者：欧启水[1] 林琳[1] 黄立东[1] 陆佩华[1] 周光炎[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025

出　　处：《上海免疫学杂志》2002年第1期12-15,共4页Shanghai Journal of Immunology

基　　金：上海市科技发展基金资助项目 ;福建省自然科研基金资助项目 ( 99C0 0 1) ;福建省教委青年科研基金资助项目 ( 99A0 5 5 )

摘　　要：为观察CIITA基因突变体对猪SLA表达的影响 ,用脂质体转染法将pcDNA3及CIITA突变体pcDNA3mCIITA2、pcDNA3mCIITA3和pcDNA3mCIITA4转染入猪血管内皮细胞系PIEC和猪B淋巴细胞系L2 3。利用流式细胞术 /RT PCR检测各种突变体对猪SLA DR、 DQ分子 /mRNA组成性和诱导性表达的影响。发现两种突变体pcDNA3mCIITA3和pcDNA3mCIITA4对PIEC/L2 3细胞SLAII类分子的表达起明显的抑制作用 ,而空载体pcDNA3和不具有起始密码子的突变体pcDNA3mCIITA2无此作用。提示pcDNA3mCIITA3和pcDNA3mCIITA4能抑制猪SLAII类分子的表达。In order to explore the effect of the mutated class II transactivator CIITA constructs on the SLA class II expression. The pcDNA3 plasmid and three CIITA mutants (including pcDNA3mCIITA2,pcDNA3mCIITA3 and pcDNA3mCIITA4) were transfected in to swine cell lines PIEC and L23 by lipofectamine. Flow cytometry and RT-PCR were used to detect the effect of the pcDNA3 plasmid and all three CIITA mutants on the SLA class II expression. It was found that pcDNA3mCIITA3 and pcDNA3mCIITA4 could suppress the inducible/constitutive expression of MHC class II genes. On the contrary,the empty vector and pcDNA3mCIITA2 had no effect on the expression of MHC class II genes. Our results indicated the constructed mutants (pcDNA3mCIITA3 and pcDNA3mCIITA4) could suppress the expression of SLA class II molecule.

关 键 词：CⅡTA 突变体构建 SLA-DR/DQ 基因转染 

分 类 号：R392.12[医药卫生—免疫学]