放射标记法研究壳聚糖与牛血清白蛋白的自组装超薄膜  

CHITOSAN/BOVINE SERUM ALBUMIN ULTRATHIN FILMS STUDIED BY RADIO-LABELED METHOD

作　　者：鲁从华[1] 罗传秋[1] 曹维孝[1] 

机构地区：[1]北京大学化学与分子工程学院高分子科学与工程系,北京100871

出　　处：《高分子学报》2002年第1期116-119,共4页Acta Polymerica Sinica

基　　金：;国家自然科学基金资助项目 (基金号 39870 2 2 7;5 0 1730 0 2 )

摘　　要：Chitosan/bovine serum albumin (BSA) ultrathin films were fabricated on modified quartz wafer through layer by layer deposition.The effects of experimental conditions, such as pH value, molecular weight of CS, ionic strength and polyanions coexisting in BSA solution, on the fabrication and the stability of films were investigated with a radio\|labeled method. The results show that the absorbed BSA is almost proportional to the number of assembly cycle except the first 2 cycles, and chitosan/BSA films are favorable to be fabricated in chitosan aq AcOH at pH=5.2 as compared with at pH=1.0. When two different molecular weight (MW) chitosans were used ( M η≈1.2×10 5, M η≈1.0×10 6), the lower MW chitosan adsorbs more BSA, but less stable ultrathin films are fabricated. Similar results were obtained that the amount of adsorbed BSA is higher but the films formed are less stable in water than in phosphate buffered saline (PBS). The adsorption of BSA is restricted greatly in the presence of poly(sodium styrene sulfonate) (PSS) mainly because of the competitive adsorption.Chitosan/bovine serum albumin (BSA) ultrathin films were fabricated on modified quartz wafer through layer-by-layer deposition. The effects of experimental conditions, such as pH value, molecular weight of CS, ionic strength and polyanions coexisting in BSA solution, on the fabrication and the stability of films were investigated with a radio-labeled method. The results show that the absorbed BSA is almost proportional to the number of assembly cycle except the first 2 cycles, and chitosan/BSA films are favorable to be fabricated in chitosan aq AcOH at pH = 5. 2 as compared with at pH = 1.0. When two different molecular weight ( MW) chitosans were used (Meta approximate to1.2 x 10(5), Meta approximate to 1.0 x 10(6)), the lower MW chitosan adsorbs more BSA, but less stable ultrathin films are fabricated. Similar results were obtained that the amount of adsorbed BSA is higher but the films formed are less stable in water than in phosphate-buffered saline (PBS). The adsorption of BSA is restricted greatly in the presence of poly(sodium styrene-sulfonate) (PSS) mainly because of the competitive adsorption.

关 键 词：壳聚糖 牛血清白蛋白 放射标记法 自组装超薄膜 生物材料 生物相容性 

分 类 号：O631[理学—高分子化学]