碱性成纤维细胞生长因子培养条件下的三维组织培养  

Three dimensional tissue culture in culture medium with rhbFGF

作　　者：王一飞[1] 戴云[1] 林剑[1] 李志英[1] 吕冀[1] 张美英[1] 李久香[1] 

机构地区：[1]暨南大学生命科学技术学院,广东广州510632

出　　处：《中国病理生理杂志》2002年第2期150-153,共4页Chinese Journal of Pathophysiology

基　　金：国家"九五"科技重点攻关项目 (No .96-C0 2 -0 1- 0 3 ) ; 广州市科委科技重点攻关项目 (No.99- 2 - 0 0 7- 0 1)

摘　　要：目的 :在含重组人碱性成纤维细胞生长因子 (rhbFGF)的细胞培养基条件下 ,研究巨噬细胞和成纤维细胞以血纤维蛋白凝块或脂肪组织为支架 ,构建三维组织模型的可能性。方法 :采用相差显微镜观察、吉姆萨 (Giem sa)染色和扫描电子显微镜观察巨噬细胞和成纤维细胞在血纤维蛋白凝块或脂肪组织支架上生长情况。结果 :巨噬细胞和成纤维细胞在血纤维蛋白凝块和脂肪组织支架上均生长良好 ,细胞间无排斥现象 ,部分细胞间建立了细胞桥连接。结论 :血纤维蛋白凝块和脂肪组织支架对两种细胞都表现出无免疫原性和很好的组织相溶性 ,细胞间不仅能够共存 。AIM: To investigate the behaviour of 3T3 fibroblast and macrophage co-culture on blood fibrin clot or adipose tissue with recombinant basic fibroblast growth factor (rhbFGF). METHODS: MTT method, inverted contrast microscopy, Giemsa staining as well as scanning electron microscope were used in the present study. RESULTS: The effect of rhbFGF on co-culture of 3T3 fibroblast and mouse macrophage on blood fibrin clot in low-serum DMEM with rhbFGF were monitored, and 3T3 fibroblast and macrophage growed well on the blood fibrin blot in low-serum DMEM with rhbFGF. CONCLUSION: The blood fibrin clot, with its low immunogenicity, could be used as a bionic support for three-dimensional tissue culture, and also a physiological carrier to distribute the growth factor rhbFGF for the cells.

关 键 词：碱性成纤维细胞生长因子 纤维蛋白 脂肪组织 巨噬细胞 组织培养 

分 类 号：R318.08[医药卫生—生物医学工程]